BTG1在HK-2和786-O细胞株中的表达及其对细胞周期和细胞凋亡的影响-东南大学学报医学版

BTG1在HK-2和786-O细胞株中的表达及其对细胞周期和细胞凋亡的影响

单位：1. 东南大学医学院, 江苏南京 210009;
2. 东南大学附属中大医院泌尿外科, 江苏南京 210009

分类号：R737.14;Q782;Q753

出版年·卷·期（页码）：2013·32·第五期（533-538）

摘要：

目的:探讨B细胞易位基因1(BTG1)在人肾小管上皮细胞株 (HK-2)和肾透明细胞腺癌细胞株(786-O)中的表达及BTG1基因对HK-2和786-O细胞周期、细胞凋亡的影响。方法:采用Western blotting方法检测BTG1蛋白在HK-2、786-O细胞株中的表达,构建BTG1表达质粒转染786-O细胞,合成BTG1干扰RNA转染HK-2细胞,通过流式细胞仪(FACS)观察BTG1对HK-2、786-O细胞周期和细胞凋亡的影响。结果:Western blotting检测显示,BTG1蛋白在HK-2中的表达高于786-O。786-O中高表达BTG1蛋白能够促进其凋亡,增加其G₀/G₁期比率;HK-2中低表达BTG1蛋白能够抑制其凋亡,减少其G₀/G₁期比率。结论:BTG1可能通过对细胞凋亡及细胞周期的调控抑制肾细胞癌的生长。

Objective: To explore the expression of B-cell translocation gene 1(BTG1) in normal renal tubule epithelial cell lines (HK-2) and renal clear cell cancer cell lines (786-O) and its influence on cell cycle and apoptosis of HK-2 and 786-O.Methods: The expression of BTG1 in HK-2 and 786-O was detected by Western blotting. The BTG1 expression plasmid was constructed and transfected into 786-O. The interference RNA of BTG1 was designed and transfected into HK-2. The effect of BTG1 on cell cycle and apoptosis of HK-2 and 786-O was observed by flow cytometry (FACS). Results: The expression of BTG1 protein was higher in HK-2 than in 786-O.High expression of BTG1 would promote cell apoptosis and increase the rate of G₀/G₁ phase in 786-O. Low expression of BTG1 would inhibit the cell apoptosis and reduce rate of G₀/G₁ phase in HK-2. Conclusion: BTG1 can inhibit the growth of renal cell carcinoma through regulation of cell cycle and apoptosis.

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