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胸腺肽α1的研究进展
作者:张鹏1 2  黄启来1  华子春1 3 
单位:1. 澳门科技大学 中医药学院, 澳门中药质量研究国家重点实验室, 澳门;
2. 江苏师范大学 生命科学学院, 江苏 徐州 221116;
3. 南京大学 医药生物技术国家重点实验室, 江苏 南京 210093
关键词:胸腺肽 临床应用 市场状况 研究进展 综述 
分类号:R979.5
出版年·卷·期(页码):2013·32·第三期(344-350)
摘要:

胸腺肽α1是一种可以用于多种疾病患者的康复和治疗的针对T细胞的免疫增强剂。作者对其在恶性肿瘤、脓毒血症、乙肝、丙肝等方面的临床应用以及基因工程生产等各方面的研究现状进行了综述,并对其应用及市场前景进行了展望。

参考文献:

[1] GOLDSTEIN A L,GUHA A,HARDY M A,et al.Purification and biological activity of thymosin,α hormone of the thymus gland[J].Proc Natl Acad Sci USA,1972,69(7):1800-1803.
[2] HOOPER J A,MCDANIEL M C,THUMRAN G B,et al.Purification and properties of bovine thymosin[J].Ann NY Acad Sci,1975,249:125-144.
[3] 宫照龙.胸腺素alpha-1基因的克隆、表达及其对淋巴细胞增殖的影响.安徽医科大学,2004.
[4] GOLDSTEIN A L,LOW T L K,MCDOO M,et al.Thymosinα1:isolation and sequence analysis of an immunologically active thymic polypeptide[J].Pro Natl Acad Sci USA,1977,74(2):725-729.
[5] LOW T L K,GOLDSTEIN A L.Thymosins:structure,function and therapeutic applications[J].Thymus,1984,6:27-42.
[6] GROTTESI A,SETTE M,PALAMARA T,et al.The conformation of peptide Tα1 in solution and in a membrane-like environment by circular dichroism and NMR spectroscopy.A possible model for its interaction with the lymphocyte membrane[J].Peptides,1998,19(10):1731-1738.
[7] SALVATI F,RASI G,PORTALONE L,et al.Combined treatment with thymosin-alphal and low-dose interferon-alpha afterifosfamide in non-small cell lung cancer:a phase-II controlled trial[J].Anticancer Res,1996,16(2):1001-1004.
[8] 余荣,孙宇,蔡庆,等.胸腺肽减轻放射性肺损伤[J].中国肺癌杂志,2011,14(3):187-193.
[9] 安彤同,刘叙仪,方健,等.胸腺肽α1治疗化疗所致神经系统副作用的初步研究[J].癌症,2004,23(11 Suppl):1428-1430.
[10] SHUQUN C,MENGCHAO W,HAN C,et al.Combination transcatheter hepatic arterial chemoembolization with thymosin alphal on recurrence prevention of hepatocellular carcinoma[J].Hepatogastroenterology,2004,51(59):1445-1447.
[11] NAYLOR P H,HADDEN J W.Preclinical studies with IRX-2 and thymosin alphal in combination therapy[J].Ann NY Acad Sci,2010,1194:162-168.
[12] SUNGARIAN A,CIELO D,SAMPATH P,et al.Potential role of thymosin-alphal adjuvant therapy for glioblastoma[J].J Oncol,2009,2009:302084.
[13] PERRUCCIO K,BONIFAZI P,TOPINI F,et al.Thymosin alphal to harness immunity to pathogens after haploidentical hematopoietic transplantation[J].Ann N Y Acad Sci,2010,1194:153-161.
[14] MAIO M,MACKIEWICZ A,TESTORI A,et al.Large randomized study of thymosin alpha 1,interferon alfa,or both in combination with dacarbazine in patients with metastatic melanoma[J].J Clin Oncol,2010,28(10):1780-1787.
[15] CHEN H,HE M Y,LI Y M.Treatment of patients with severe sepsis using ulinastatin and thymosin alphal:a prospective,randomized,controlled pilot study[J].Chin Med J (Engl),2009,122(8):883-888.
[16] 黄顺伟,管向东,陈娟,等.乌司他丁联合胸腺肽α1改善脓毒症患者免疫功能的作用机制研究[J].中国病理生理杂志,2009,25(11):2168-2172.
[17] LI Y M,CHEN H,LI X,et al.A new immunomodulatory therapy for severe sepsis:ulinastatin plus thymosin alpha 1[J].J Intensive Care Med,2009,24(1):47-53.
[18] 黄顺伟,管向东,陈娟,等.创伤性严重脓毒症和多器官功能障碍综合征免疫调理治疗的临床研究和远期评价[J].中国危重病医学,2006,18(11):653-656.
[19] ZHANG Y,CHEN H,LI Y M,et al.Thymosin alphal- and ulinastatin-based immunomodulatory strategy for sepsis arising from intra-abdominal infection due to carbapenem-resistant bacteria[J].J Infect Dis,2008,198(5):723-730.
[20] CHIEN R N,LIAW Y F,CHEN T C,et al.Efficacy of thymosin alphal in patients with chronic hepatitis B:a randomized,controlled trial[J].Hepatology,1998,27(5):1383-1387.
[21] RASI G,TERZOLI E,IZZO F,et al.Combined treatment with thymosin-alphal and low dose interferon-alpha after dacarbazine in advanced melanoma[J].Melanoma Res,2000,10(2):189-192.
[22] LIM S G,WAI C T,LEE Y M,et al.A randomized,placebo-controlled trial of thymosin-alphal and lymphoblastoid interferon for HBeAg-positive chronic hepatitis B[J].Antivir Ther,2006,11(2):245-253.
[23] 毛海鹰,石统东.干扰素联合胸腺肽α1治疗e抗原阳性慢性乙型肝炎的荟萃分析[J].中国肝病杂志,2011,19(1):29-33.
[24] JIANG Y F,MA Z H,ZHAO P W,et al.Effect of thymosin-α(1) on T-helper 1 cell and T-helper 2 cell cytokine synthesis in patients with hepatitis B virus e antigen-positive chronic hepatitis B[J].J Int Med Res,2010,38(6):2053-2062.
[25] LOGGI E,GRAMENZI A,MARGOTTI M,et al.In vitro effect of thymosin-alphal and interferon-alpha on Th1 and Th2 cytokine synthesis in patients with eAg-negative chronic hepatitis B[J].J Viral Hepat,2008,15(6):442-448.
[26] ANDREONE P,CURSARO C,GRAMENZI A,et al.A randomized controlled trial of thymosin-alphal versus interferon alfa treatment in patients with hepatitis B e antigen antibody-and hepatitis B virus DNA--positive chronic hepatitis B[J].Hepatology,1996,24(4):774-777.
[27] 蒋滨.胸腺肽联合抗乙肝免疫核糖核酸治疗慢性乙型肝炎的疗效观察[J].现代医学,2009,37(4):282-283.
[28] GRANDINI E,CANNOLETTA F,SCUTERI A,et al.Immunological modifications during treatment with thymosin alphal plus antiviral,therapy in chronic hepatitis C[J].Ann NY Acad Sci,2010,1194:147-152.
[29] SUGAHARA S,ICHIDA T,YAMAGIWA S,et al.Thymosin-alphal increases intrahepatic NKT cells and CTLs in patients with chronic hepatitis B[J].Hepatol Res,2002,24(4):346-354.
[30] ANDREONE P,CURSARO C,GRAMENZI A,et al.In vitro effect of thymosin-alphal and interferon-alpha on Th1 and Th2 cytokine synthesis in patients with chronic hepatitis C[J].J Viral Hepat,2001,8(3):194-201.
[31] SHERMAN K E,SJOGREN M,CREAGER R L,et al.Combination therapy with thymosin alphal and interferon for the treatment ofchronic hepatitis C infection:a randomized,placebo-controlled double-blind trial[J].Hepatology,1998,27(4):1128-1135.
[32] 王琳,郭成山,侯明,等.胸腺肽α1联合大剂量地塞米松治疗慢性特发性血小板减少性紫癜的疗效分析[J].中华内科学杂志,2007,46(4):274-276.
[33] 马丽娜,陈新月,陈杰,等.胸腺肽α1辅助治疗肝硬化自发性腹膜炎[J].中华肝脏杂志,2007,15(3):225.
[34] 黄登鹏,杨鸣,彭卫平,等.胸腺肽α1对危重病人气管切开肺部感染的防治作用[J].南方医科大学学报,2006,26(1):128-129.
[35] WANG X,LI W,NIU C,et al.Thymosin alpha 1 is associated with improved cellular immunity and reduced infection rate in severe acute pancreatitis patients in a double-blind randomized control study[J].Inflammation,2011,34(3):198-202.
[36] 郑碧霞,程德云,徐刚,等.胸腺肽α1对慢性阻塞性肺疾病急性加重的预防作用[J].四川大学学报,2008,39 (4):588-590.
[37] 顾小军,沈玲,黄英,等.胸腺肽联合狂犬病疫苗接种对机体产生γ-干扰素、白细胞介素-2和中和抗体影响的研究[J].东南大学学报:医学版,2012,31(5):550-554.
[38] ZHANG H Y,CHEN P F,XU J M,et al.Separation and purification of Escherichia coli-expressed human thymosin-α1 using affinity chromatography and high-performance liquid chromatography[J].Protein Expr Purif,2011,77(2):140-145.
[39] CHEN P F,ZHANG H Y,FU G F,et al.Overexpression of soluble human thymosin alpha 1 in Escherichia coli[J].Acta Biochim Biophys Sin (Shanghai),2005,37(2):147-151.
[40] LI W,SONG L,WU S,et al.Expression,purification and characterization of a novel soluble human thymosin alphal concatemer exhibited a stronger stimulation on mice lymphocytes proliferation and higher anti-tumor activity[J].Int J Biol Sci,2011,7(5):618-628.
[41] CHEN Y,ZHAO L,SHEN G,et al.Expression and analysis of thymosin alphal concatemer in Escherichia coli[J].Biotechnol Appl Biochem,2008,49(Pt 1):51-56.
[42] ESIPOV R S,STEPANENKO V N,BEYRAKHOVA K A,et al.Production of thymosin alphal via non-enzymatic acetylation of the recombinant precursor[J].Biotechnol Appl Biochem,2010,56(1):17-25.
[43] 刘先俊,刘方欣,黎波,等.胸腺素α1与复合α干扰素融合蛋白的表达及其生物学活性[J].生物工程学报,2008,24(7):1168-1173.
[44] KOROBKO V G,BOLDYREVA E F,FILIPPOV S A,et al.Synthesis of an artificial gene coding for thymosin alphal and its expression in Escherichia coli as a hybrid with human tumor necrosis factor[J].Bioorg Khim,1992,18(5):646-659.
[45] SHEN Q,TIAN R,MA W,et al.Construction and expression of a new fusion protein,thymosin alphal-cBLyS,E.coli.[J].Biotechnol Lett,2005,7(3):143-148.
[46] FEDOROV T V,KOROBOV V I,NAZAROV V G,et al.urification of recombinant proteins with an example of tumor necrosis factor thymosin-alphal[J].Prikl Biokhim Mikrobiol,2010,6(2):243-247.
[47] REN Y,YAO X,DAI H,et al.roduction of Nα-acetylated thymosin α1 in Escherichia coli[J].Microb Cell Fact,2011,2:10-26.
[48] CHEN J H,ZHANG X G,JIANG Y T,et al.ioactivity and pharmacokinetics of two human serum albumin-thymosin alphal-fusion proteins,rHSA-Talphal and rHSA-L-Talphal,expressed in recombinant ichia pastoris[J].Cancer Immunol Immunother,2010,9(9):1335-1345.
[49] GAO D,ZHANG X,ZHANG J,et al.Expression of thymosin alphal-thymopentin fusion peptide in Pichia pastoris and its characterization[J].Arch Pharm Res,2008,31(11):1471-1476.
[50] CHEN F,CHEN X M,CHEN Z,et al.Construction and application of a yeast expression system for thymosin alphal[J].Biocell,2005,29(3):253-259.
[51] 黄雯,李兆育,金礼吉,等.人胸腺素α1基因在毕赤酵母中的融合表达[J].遗传,2002,24(6):679-683.
[52] YANG Y F,YUAN H Y,LIU N S,et al.Construction,expression and characterization of human interferon alpha2b-(G4S)n- thymosin alphal fusion proteins in Pichia pastoris[J].World J Gastroenterol,2005,11(17):2597-2602.
[53] CHEN Y,WANG A,ZHAO L,et al.Expression of thymosin alphal concatemer in transgenic tomato (Solanum lycopersicum) fruits[J].Biotechnol Appl Biochem,2009,52(4):303-312.

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