电感耦合等离子体发射光谱法测定尿铜的评价-东南大学学报医学版

电感耦合等离子体发射光谱法测定尿铜的评价

单位：南京医科大学附属南京儿童医院, 江苏南京 210008

分类号：R446.12

出版年·卷·期（页码）：2013·32·第三期（327-329）

摘要：

目的:评价电感耦合等离子体发射光谱法(ICP-AES)测定尿铜。方法:将尿液标本用去离子水稀释5倍,使用JY ULTIMA-2型电感耦合等离子体发射光谱仪,在波长324.754nm下测定尿铜标本。结果:1个月内对3个浓度水平的尿铜标本各检测分批,每批重复测定3次。批内CV 4.2%~4.6%,批间CV 4.8%~5.0%,总CV 4.6%~4.9%,方法的精密度1.57%~1.66%,回收率97.4%~98.8%,尿铜元素的线性相关系数r=0.9998。结论:ICP-AES检测尿铜精密度和准确度高,可作为检测尿铜的常规方法。

Objective: The evaluation of methodology measuring urinary copper using ICP-AES. Method: The urine specimen were diluted 5 times with deionized wate,followed by detection using the best-working-state JY ULTIMA-2 ICP-AES on the condition of 324.754nm. Result: Three urine specimens were detected in different concentration batched three within one month.Each time,they were detected three times.The range of batch inside was CV 4.2%~4.6%,between batches was CV 4.8%~5.0% and the total CV 4.6%~4.9%.The method degree of precision was 1.57%~1.66%.The standardized recovery was 97.4%~98.8%.The linear correlation coefficient of the urinary copper was r=0.9998. Conclusion: Laboratory tests suggest that the method using ICP-AES to detect urinary copper does not have systemic errores.This method is accurate and reliable which can be applied as a conventional way to detect urinary copper.

参考文献：

[1] ALA A,SCHILSKY M L.Wilson disease:pathophysiology diagnosis treatment and screening[J].Clin Liver Dis,2004,8:787-805.
[2] LANGER C,DENK H.Wilson disease:virchows arch[J]. 2004,445:111-118.
[3] GIACCHIO R,MARAZZI M G,BARABINO A,et al.Syndromic variability of Wilson's disease in Children[J].Ital J Gastmenterol Hepatol,1997,29:155-161.
[4] 郁飞,李萌,郭敏.人巨细胞病毒感染新生儿血液微量元素的检测[J].东南大学学报:医学版,2010,29(1):51-53.
[5] International Union of Pure and Applied Chemistry (IUPAC):Analitical Chemistry Division.Nomenclature,symbols,units and their usage in spectrochemical analysis-V radiation sources[J].Pure Appl Chem,1981,19(6):395-412.
[6] PYBUS J,FELDMAN F J,BOWER G N Jr.Measurement of total calcium serum by atomic abomic absorption spectrophotometry,with use of a strontium internal reference[J].Clin Chem,1970,16(3):998-1007.
[7] 马怀安,王清涛,王金英,等.血清钙测量参考方法的复现及方法学评价[J].中华检验医学杂志,2009,32(5):526-530.

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