PDGF调控子宫内膜异位症在位内膜间质细胞中cofilin-1基因的表达及意义-东南大学学报医学版

PDGF调控子宫内膜异位症在位内膜间质细胞中cofilin-1基因的表达及意义

单位：中国医科大学附属盛京医院, 辽宁沈阳 110004

分类号：R711.71

出版年·卷·期（页码）：2013·32·第三期（302-305）

摘要：

目的:探讨cofilin-1基因(CFL1)在子宫内膜异位症在位内膜间质细胞(ESC)异常表达与血小板源性生长因子(PDGF)的关系。方法:获取在位子宫内膜组织,分离培养ESC,以不同的时间和不同浓度的PDGF作用ESC,实时定量PCR检测在PDGF刺激前后CFL1的表达。结果:10 ng·ml^-1 PDGF作用3、6、12h,CFL1 mRNA表达均增高,其中作用6h和12h表达增高有统计学意义(P<0.05);以1、10、100ng·ml^-1的PDGF分别作用ESC 6h,CFL1 mRNA表达均增高,有统计学意义(P<0.05)。CFL1在ESC 中的表达对PDGF有时间依赖性和剂量依赖性。结论:PDGF是子宫内膜异位症在位内膜间质细胞CFL1表达的强诱导剂。

Objective: To investigate whether there is a correlation between the expression of cofilin(CFL1) of eutopic endometrium stromal cells (ESC) and PDGF stimulation. Methods: ESCs from twelve patients with advanced ovarian EMs were isolated.In a time-or dose-dependent manner,PDGF was added into culture medium depleted of fetal calf serum.The expression of CFL1 before and after PDGF treatment was detected by real time PCR. Results: PDGF induced the expression of CFL1 in ESC significantly in a time-or dose-dependent manner.ESC were treated with 10 ng·ml^-1 PDGF for 0,3,6,and 12h,CFL1 mRNA expression were increased, when compared with non-treated ESC 6 h and 12 h had statistical significance(P<0.05).ESCs were treated with 0,1,10,and 100 ng·ml^-1 PDGF for 6h,CFL1 mRNA expression were increased with statistical significance (P<0.05). Conclusions: PDGF can induce CFL1 expression in ESC.

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