目的:比较已临床确诊的布鲁杆菌病患者血培养样本ATB NEW细菌鉴定仪、手工鉴定和VITEK 2细菌鉴定仪的细菌鉴定结果,说明ATB NEW 对布鲁杆菌鉴定的局限性。方法:按照3种鉴定方法的标准操作规程对900例临床确诊的布鲁杆菌病病人血标本进行细菌培养和鉴定;对ATB NEW细菌鉴定仪鉴定为人苍白杆菌的标本用手工方法复检;同时随机抽取10例标本用VITEK 2细菌鉴定仪进行确认。结果:170例血培养阳性标本用ATB NEW细菌鉴定仪鉴定为人苍白杆菌,用手工法鉴定为布鲁杆菌,10例随机样本VITEK 2鉴定为马耳他布鲁杆菌。结论:ATB NEW细菌鉴定仪鉴定为人苍白杆菌的标本应采用其它方法进行复检,以减少误诊。

[1] 金宁一,胡仲明,冯书章.新编人兽共患病学[M].北京:科学出版社,2007:493.
[2] DOGANAY M,AYGEN B.Human brucellosis:an overview[J].Int J Infect Dis,2003,7(3):173-181.
[3] 肖东楼.布鲁氏菌病防治手册[M].北京:人民卫生出版社,2008:115-116.
[4] GROSS P A,FRYDA R,REILLY K.Comparative evaluation of different types of blood culture media for isolation of aerobes[J].J Clin Microbiol,1977,6:362-366.
[5] CORBEL M J.Brucellosis:an overview[J].Emerg Infect Dis,1997,3:213-221.
[6] NIELSEN K H,DUNCAN J R,STEMSHORN B W.Non-specific reactions to the Brucella abortus SAT[J].Vet Rec,1985,116:550-552.
[7] JACQUES I,OLIVIER-BERNARDIN V,DUBRAY G.Efficacy of ELISA compared to conventional tests(RBPT and CFT) for the diagnosis of Brucella melitensis infection in sheep[J].Vet Microbiol,1998,64:61-73.