非小细胞肺癌组织中PTEN基因与Ki67蛋白的表达及其意义-东南大学学报医学版

非小细胞肺癌组织中PTEN基因与Ki67蛋白的表达及其意义

单位：南京医科大学附属无锡市人民医院心胸外科,江苏无锡 214023

分类号：Q786;R734.2

出版年·卷·期（页码）：2013·32·第二期（141-145）

摘要：

目的:探讨第10染色体同源丢失性磷酸酶-张力蛋白酶(PTEN)基因、Ki67蛋白在非小细胞肺癌(NSCLC)组织中的表达及其临床意义。方法:用免疫组化方法检测67例NSCLC组织以及41例癌旁正常肺组织中PTEN、Ki67蛋白的表达,并分析其与各临床病理指标及细胞增殖之间的相关性。结果:PTEN在正常肺组织中阳性表达率显著高于NSCLC组织(P<0.05),PTEN表达与组织类型、细胞分化程度、淋巴结转移密切相关(P<0.05);肺癌组织中Ki67的过度表达与肺癌的临床分期、淋巴结转移相关(P<0.05);PTEN表达与Ki67表达负相关(r=-0.239,P<0.05)。结论:PTEN蛋白表达的缺失与NSCLC的恶性侵袭有关。联合检测PTEN与Ki67的表达有助于判断NSCLC的预后。

Objective: To study the expression of gene PTEN,Ki67 protein in non-small cell lung cancer(NSCLC) and the relationships with cell proliferation.Methods: The protein expressions of PTEN and Ki67 were evaluated by immunohistochemistry in 67 NSCLC patients and 41 adjacent normal lung tissue.The correlations with histopathologic and cell proliferation were also analyzed.Results: The positive expression of PTEN in adjacent normal lung tissues was higher than NSCLC(P<0.05).The expression of PTEN was strongly correlated with tumor histology, histologic grade and lymph node metastasis(P<0.05).The overexpression of Ki67 was associated with TNM stage and lymph node metastasis(P<0.05).PTEN expression was negatively related to the overexpression of Ki67(P<0.05).Conclusion: The loss of PTEN expression plays different roles in tumorigenesis of NSCLC and in relation with high aggressiveness of the tumors. The loss of PTEN expression and overexpression of Ki67 can estimate the prognosis of NSCLC.

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