吡格列酮对胶质瘤细胞生长的抑制研究-东南大学学报医学版

吡格列酮对胶质瘤细胞生长的抑制研究

单位：盐城市第一人民医院神经外科, 江苏盐城 224001

分类号：R739.41;R-33

出版年·卷·期（页码）：2013·32·第一期（42-45）

摘要：

目的:观察PPARγ激动剂吡格列酮(pioglitazone)对胶质瘤细胞生长、迁移、凋亡等生物学行为的影响,并研究其相关分子作用机制。方法:CCK-8法、细胞划痕试验、TUNEL法检测吡格列酮对胶质瘤细胞增殖、侵袭和凋亡的影响;Western Blot法检测吡格列酮刺激后对胶质瘤细胞中相关基因表达的影响。结果:吡格列酮抑制胶质瘤细胞生长,降低细胞侵袭能力并诱导其凋亡。吡格列酮能够下调β连环蛋白(β-catenin)、MMP-2蛋白表达,上调Bad、Bax蛋白表达水平。转染β-catenin siRNA后检测发现MMP-2表达降低,Bad、Bax表达上调。结论:吡格列酮抑制胶质瘤细胞增殖和迁移,促进细胞凋亡,具有抗胶质瘤的功效。吡格列酮可能通过β-catenin介导抑制胶质瘤细胞生长。

Objective: To investigate the effects of peroxisome proliferator-activated receptor γ(PPARγ) agonist pioglitazone on the growth, invasion and apoptosis of glioma cells, and the possible mechanism. Methods: The effects of pioglitazone in different concentrations and in different action times on the growth of glioma cells were examined by CCK-8 analysis,the cell-migration by monolayer wound healing assay after pioglitazone stimulation and the apoptosis of cells under pioglitazone-treated by TUNEL method. Western Blot assay was used to evaluate the possible mechanism involve in the effect of pioglitazone. Results: Pioglitazone could effectively lead to glioma cells growth suppression, invasion reduction, and apoptosis increase. Western Blot assay showed that Bax and Bad were up-regulated and MMP-2 was down-regulated in response to pioglitazone treatment. Pioglitazone induced β-catenin repression in does-dependent and time-dependent manner. Conclusion: PPARγ agonist pioglitazone which can decrease glioma cells proliferation, migration and promote cell apoptosis displays antineoplastic effects on glioma cells. pioglitazone inhibits glioma cells growth mediated by β-catenin.

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