锌-<em>α</em>2-糖蛋白对结直肠癌细胞株LoVo生物学行为的影响-东南大学学报医学版

锌-α2-糖蛋白对结直肠癌细胞株LoVo生物学行为的影响

单位：1. 东南大学医学院, 江苏南京 210009;
2. 东南大学附属第二医院, 江苏南京 210003

分类号：R735.35;R735.37

出版年·卷·期（页码）：2013·32·第一期（14-18）

摘要：

目的:初步探讨锌-α2-糖蛋白(ZAG)对结直肠癌细胞LoVo的生物学行为的影响。方法:构建和应用ZAG过表达质粒pGCMV/EGFP/Neo/ZAG转染结直肠癌细胞LoVo,免疫组化检测ZAG的表达变化,MTT法检测转染前后细胞增殖能力的变化以及细胞的克隆形成能力和侵袭力的改变。结果:过表达质粒pGCMV/EGFP/Neo/ZAG可成功诱导结直肠癌细胞LoVo表达ZAG,同时其细胞增殖力受到抑制、克隆形成率降低以及细胞的侵袭能力明显降低。结论:结直肠癌细胞LoVo的生物学行为的改变可能与ZAG的过表达相关,其在结直肠癌的发生发展过程发挥抑癌的生物学效应。

Objective: To explore the affection of adipokine zinc-α2-glycoprotein( ZAG) on the biological behavior in colorectal cancer LoVo cells. Methods: ZAG overexpression plasmid pGCMV/EGFP/Neo/ZAG was constructed and transfected into colorectal cancer LoVo cells, and the protein's expression of ZAG was detected by immunohistochemical. MTT assay was used to detect cell proliferation and the change of colony formation and invasive ability. Results: The overexpression of ZAG in colorectal cancer LoVo cells was induced, and the cell proliferation was significantly inhibited. Meanwhile, colony formation rate and cell invasion ability were significantly reduced comparing to the no-transfection-Lovo cells. Conclusion: The changes in the biological behavior of colorectal cancer LoVo cells may be correlated with the overexpression ZAG protein, which may have a tumor suppressor effect in the emergence and development of colorectal cancer.

参考文献：

[1] BURGI W,SCHMID K.Preparation and properties of Zn-alpha 2-glycoprotein of normal human plasma[J].J Biol Chem,1961,236(4):1066-1074.
[2] 龚凤英,邓洁英.锌α2糖蛋白——一种新型的脂肪代谢调节因子[J].国际内科学杂志,2007,34(5):297-300.
[3] HALE L P,PRICE D T,SANCHEZ L M,et a1.Zinc alpha-2-glyco-protein is expressed by malignant prostatic epithelium and may serve as a potential serum marker for prostate cancer[J].Clin Cancer Res,2001,7(4):846-853.
[4] DESCAZEAUD A,TAILLE Y,ALLORY,et al.Characterization of ZAG protein expression in prostate cancer using a semi-automated microscope system[J].Prostate, 2006,66(10):1037-1043.
[5] PISCHON T,LAHMANN P H,BOEING H,et al.Body size and risk of colon and rectal cancer in the European Prospective Investigation Into Cancer and Nutrition[J].J Natl Cancer Inst,2006,98(13):920-931.
[6] 马爱迪,王彩莲,闵捷,等.血清脂质水平与结直肠癌远处转移的相关性分析[J].东南大学学报:医学版,2012,31(4):471-473.
[7] KONG B,MICHALSKI C W,HONG X,et al.AZGP1 is a tumor suppressor in pancreatic cancer inducing mesenchymal-to-epithelial transdifferentiation by inhibiting TGF-β-mediated ERK signaling[J].Oncogene,2010,29(37):5146-5158.

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