USPIO增强MRI检测人野生型p53基因转染动脉粥样硬化易损斑块的研究-东南大学学报医学版

USPIO增强MRI检测人野生型p53基因转染动脉粥样硬化易损斑块的研究

单位：1. 徐州医学院心血管病研究所, 江苏徐州 221000;2. 徐州医学院第二附属医院, 江苏徐州 221000

分类号：R541.4

出版年·卷·期（页码）：2012·31·第六期（681-687）

摘要：

目的:探讨超微超顺磁氧化铁颗粒(USPIO)增强MRI检测人野生型p53基因转染动脉粥样硬化(AS)易损斑块(VP)的可行性及有效性。方法:选取健康雄性新西兰大白兔40只,通过球囊导管损伤腹主动脉内膜+高脂饮食饲养+人野生型p53基因转染腹主动脉斑块+药物触发方法,建立VP模型;观察USPIO增强前后AS斑块MRI信号表现,分析易损斑块病理组织学特点与MRI影像之间的相关性。结果:38只新西兰大白兔建立动物模型成功。MRI显示,腹主动脉斑块呈扁平状或新月状向管腔内突起,导致管腔狭窄,部分伴斑块内出血,血栓形成。USPIO增强T2^*WI表现为斑块中央信号减低,其负性强化峰值出现在注射对比剂后96h;斑块增强MRI序列与HE染色病理学结果之间有良好的相关性。结论:USPIO增强MRI可通过特异性识别VP中的巨噬细胞浸润而发现VP,是一项对AS病变诊断及斑块稳定性的早期评估有前途的影像技术。

Objective: To investigate the feasibility and value of ultra small super paramagnetic iron oxide(USPIO)-enhanced magnetic resonance imaging(MRI) in detection of human wild-type p53 gene transfection of atherosclerotic vulnerable plaque.Methods:Forty rabbits underwent aortic balloon injury combined with cholesterol diet, then the abdominal aortic plagues were injected with human wild-type p53 gene, in this way, vulnerable plague model were established. The plaques signal change were analyzed before and after the injection of USPIO, a correlative study was done between MRI and pathological findings in vulnerable plague. Results: Animal model were successfully established with 38 New Zealand white rabbits,within the abnormal of lumen, the flat,crescent-shaped,protrusion of the plaque,which leaded stenosis of lumen were found,and some with intraplague hemorrhage or thrombosis in MRI images; The signal of USPIO enhanced T2^* WI in central area of the plaques decreased and the peak of the negtive enhancement occurred 96 h after the injection of contrast agent. The matching of contrast-enhanced MR imaging and HE staining of pathological results were showed good correlation in plague. Conclusions: USPIO-enhanced MRI can specifically recognize the macrophage infiltration in VP, then found it. It's a promising imaging technique about diagnosis of AS and early assessment of plaque stability.

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