Objective: To investigate the feasibility and value of ultra small super paramagnetic iron oxide(USPIO)-enhanced magnetic resonance imaging(MRI) in detection of human wild-type p53 gene transfection of atherosclerotic vulnerable plaque.Methods:Forty rabbits underwent aortic balloon injury combined with cholesterol diet, then the abdominal aortic plagues were injected with human wild-type p53 gene, in this way, vulnerable plague model were established. The plaques signal change were analyzed before and after the injection of USPIO, a correlative study was done between MRI and pathological findings in vulnerable plague. Results: Animal model were successfully established with 38 New Zealand white rabbits,within the abnormal of lumen, the flat,crescent-shaped,protrusion of the plaque,which leaded stenosis of lumen were found,and some with intraplague hemorrhage or thrombosis in MRI images; The signal of USPIO enhanced T2* WI in central area of the plaques decreased and the peak of the negtive enhancement occurred 96 h after the injection of contrast agent. The matching of contrast-enhanced MR imaging and HE staining of pathological results were showed good correlation in plague. Conclusions: USPIO-enhanced MRI can specifically recognize the macrophage infiltration in VP, then found it. It's a promising imaging technique about diagnosis of AS and early assessment of plaque stability. |
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