Objective To investigate the influence of lipopolysaccharide(LPS) on the collagen synthesis of normal human skin fibroblasts and its relation with wound healing. Methods Normal skin fibroblasts were exposed to LPS for a certain period of time. Then the collagen synthesis of fibroblasts in culture medium was measured with the method of pepsin digestion after incorporation of 3H-proline into stable, single-layered, confluent fibroblasts. Results Collagen synthesis of normal skin fibroblasts increased when challenged with LPS at the concentration of 0.005μg/ml, and the increase showed a concentration dependent manner; However, when the concentration of LPS was 0.5μg/ml，the influence of LPS on the collagen synthesis of normal skin fibroblasts began to decrease; When the concentration of LPS reached 1.0μg/ml, collagen synthesis of fibroblasts were inhibited. Conclusion LPS could promote collagen synthesis of normal human skin fibroblasts within a certain extent, suggesting that LPS might play an important role in wound healing.