脂多糖对正常人皮肤成纤维细胞胶原合成的影响-东南大学学报医学版

脂多糖对正常人皮肤成纤维细胞胶原合成的影响

单位：解放军第二五一医院急诊科,河北张家口 075000

分类号：Q516; R335

出版年·卷·期（页码）：2012·31·第五期（613-615）

摘要：

目的:研究脂多糖(lipopolysaccharide, LPS)对正常人皮肤成纤维细胞胶原合成的影响,以阐明LPS在皮肤创伤愈合中的可能作用。方法:体外培养正常人皮肤成纤维细胞,采用³H-脯氨酸掺入法观察不同浓度(0、0.005、0.01、0.05、0.1、0.5和1.0 μg·ml^-1)的LPS对成纤维细胞胶原合成的影响。结果:LPS刺激浓度在0.005 μg·ml^-1时,皮肤成纤维细胞胶原蛋白合成增加;随着刺激浓度增加,刺激作用增强;但当LPS浓度为0.5 μg·ml^-1时,促胶原合成作用开始降低;当浓度达到1.0 μg·ml^-1时则呈现抑制效应。结论:在一定浓度范围内LPS促进成纤维细胞胶原合成,表明LPS在皮肤创伤愈合中可能具有重要作用。

Objective: To investigate the influence of lipopolysaccharide(LPS) on the collagen synthesis of normal human skin fibroblasts and its relation with wound healing. Methods: Normal skin fibroblasts were exposed to LPS for a certain period of time. Then the collagen synthesis of fibroblasts in culture medium was measured with the method of pepsin digestion after incorporation of ³H-proline into stable, single-layered, confluent fibroblasts. Results: Collagen synthesis of normal skin fibroblasts increased when challenged with LPS at the concentration of 0.005 μg·ml^-1, and the increase showed a concentration dependent manner. However, when the concentration of LPS was 0.5 μg·ml^-1,the influence of LPS on the collagen synthesis of normal skin fibroblasts began to decrease; when the concentration of LPS reached 1.0 μg·ml^-1, collagen synthesis of fibroblasts were inhibited. Conclusion: LPS could promote collagen synthesis of normal human skin fibroblasts within a certain extent, suggesting that LPS might play an important role in wound healing.

参考文献：

[1] KOHYAMA T,LIU X,WEN F Q,et al.Cytokines modulate cilomilast response in lung fibroblasts[J].Clin Immunol,2004,111(3):297-302.
[2] 杨红明,付小兵,柴家科,等.脂多糖对皮肤纤维母细胞和表皮细胞的影响[J].解放军医学杂志,2000,25(6):428-429.
[3] BABU M,DIEGELMANN R,OLIVER N.Fibronectin is overproduced by keloid fibroblasts during abnormal wound healing[J].Mol Cell Biol,1989,9(4):1642-1650.
[4] YANG H M,KANKO M,HE C,et al.Effect of a lipopolysaccharide from E.coli on the proliferation of fibroblasts and keratinocytes in vitro[J].Phytother Res,2002,16(1):43-47.
[5] 董波,陈肖华,张浩,等.一种改进的用于测定细胞周期的细胞制备方法[J].军事医学科学院院刊,2002,26(2):124-129.
[6] 倪杰,戴厚永,郑敏,等.抗体芯片在糖尿病肾病大鼠尿液细胞因子同步检测中的应用[J].东南大学学报:医学版,2012,31(3):249-253.
[7] 李从青,姚洁,刘长明,等.内毒素对妊娠期糖尿病患者外周血单核细胞TLR-4 mRNA及NFκ-B mRNA表达的影响[J].安徽医科大学学报,2011,11(3):50-53.
[8] 徐荔,何小鹃,柴旺,等.黄芪多糖对内毒素诱导巨噬细胞产生TNF-α、IL-1β及NO的影响[J].中国中医基础医学杂志,2011,17(5):62-63.

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