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TGF-β/TβR1通路通过上调MMP2表达促进头颈鳞状细胞癌侵袭
作者:代炜1  姚远2  李彦姝3  张红艳3  李妍3  秦兴军1 
单位:1. 中国医科大学口腔医学院口腔颌面外科,口腔颌面-头颈肿瘤外科,辽宁 沈阳 110002;
2. 辽宁省人民医院消化内科,辽宁 沈阳 110016;
3. 中国医科大学细胞生物学教研室,细胞生物学卫生部重点实验室,辽宁 沈阳 110001
关键词:转化生长因子受体1 基质金属蛋白酶-2 稳转细胞系 侵袭转移 
分类号:R739.81
出版年·卷·期(页码):2012·31·第五期(555-558)
摘要:

目的:研究转化生长因子受体1(TβR1)与基质金属蛋白酶-2(MMP2)在头颈鳞状细胞癌中的关系及其对肿瘤细胞侵袭转移机制的调控。方法:构建稳定过表达TβR1的UM-SCC-23细胞系,SDS-PAGE电泳和蛋白质印迹法鉴定细胞系构建。利用Real-time PCR和蛋白质印迹法检测MMP2在稳转细胞系中的表达。细胞侵袭试验检测稳转TβR1的UM-SCC-23细胞系侵袭能力。结果:成功构建了稳定表达TβR1的UM-SCC-23,MMP2在TβR1过表达的UM-SCC-23细胞中基因和蛋白水平表达均增高,稳定表达了TβR1的UM-SCC-23细胞侵袭能力增强。结论:TGF-β/TβR1通路能够上调MMP2表达,促进头颈鳞状细胞癌侵袭能力增强。

Objective: To investigate the relationship between transforming growth factor-beta receptor type-1 (TβR1) and matrix metallopeptidase 2(MMP2) in head and neck squamous cell carcinoma,and to examine the influence of TβR1 and MMP2 on the regulation of cancer cell invasion. Methods: We generated a stable UM-SCC-23 cell line expressing His-tagged TβR1, clonal cell lines were selected based on the level of TβR1 by SDS-PAGE and Western blotting analysis. Expression of MMP2 was detected by real-time PCR and Western blotting in the stable cell lines. TβR1 overexpression promoted UM-SCC-23 cell invasion by cell invasion assay. Results: Stable transfection UM-SCC-23-TβR1 cell model has been successfully established. MMP2 was significantly increased in stable transfection UM-SCC-23-TβR1 cell lines compared to the control cell lines. UM-SCC-23 cancer cell lines with a stable expression of TβR1 exhibit an increase in cell invasion abilities. Conclusion: This study suggests an additional role of TGF-β/TβR1 and MMP2 in oral squamous cell carcinoma(OSCC). TβR1 upregulate the expression of MMP2 and promote the invasion of OSCC cancer cell.

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