目的:探讨人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,HUcMSCs),对新生未成熟鼠脑室周围白质软化灶受损神经细胞的保护作用。方法:建立新生大鼠PVL(periventricular leukomalacia)动物模型,造模后随机分为两组。于第5天(P5d)分别给两组大鼠腹腔注射5-溴脱氧尿核苷(5-bromodeoxyuridine,BrdU)标记的第二代HUcMSCs[每只1×107(0.05 ml)-1]或0.05 ml PBS无菌溶液。24 h后(P6d)处死一组大鼠,行BrdU染色观察HUcMSCs在鼠脑内的迁移;另一组于移植HUcMSCs后14 d(P19d)行微型MRI(MicroMRI)扫描观察受损区域修复程度,髓磷脂碱性蛋白(myelin basic protein,MBP)染色观察移植HUcMSCs对大鼠脑室周围脑白质损伤的保护。结果:HUcMSCs在宿主体内明显向侧脑室周围白质损伤区域迁移,且脑室周围存活少突胶质细胞数量明显多于注射PBS组,微型MRI扫描观察到HUcMSCs可缩小受损脑白质病灶,远期神经行为学观察到HUcMSCs移植组神经行为学高于PBS组(P<0.05)。结论:HUcMSCs对新生大鼠PVL具有神经保护作用,可缩小病变范围。 |
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