木犀草素抑制人肺腺癌A549细胞株的运动及其机制研究-东南大学学报医学版

木犀草素抑制人肺腺癌A549细胞株的运动及其机制研究

单位：1. 澳门科技大学中医药学院澳门药物与健康应用研究所中药质量研究国家重点实验室,澳门;
2. 南京大学医药生物技术国家重点实验室,江苏南京 210093

分类号：R285.5; R734.2

出版年·卷·期（页码）：2012·31·第四期（415-418）

摘要：

目的:研究木犀草素(luteolin)对人肺腺癌A549细胞株运动的影响,并探讨其作用的相关机制。方法:MTT法检测木犀草素对人肺腺癌A549细胞株细胞活力的影响;伤口愈合实验观察木犀草素对A549细胞运动的影响;蛋白水平检测局部黏着斑激酶(FAK)及其磷酸化FAK的表达变化。结果:木犀草素作用于A549细胞后,呈剂量依赖性抑制A549细胞的增殖,IC₅₀约为54.4 μmol·L^-1;木犀草素在15 μmol·L^-1剂量下,24 h和48 h后显著抑制A549细胞向损伤区域的迁移能力;蛋白水平检测发现木犀草素剂量依赖性地降低FAK磷酸化的水平,此外,FAK总蛋白的含量也有所下降。结论:木犀草素在对细胞生长无显著抑制效应的剂量下,通过作用于FAK这一关键分子抑制肿瘤细胞的运动。

Objective: To study the effect of luteolin on the motility of human lung adenocarcinoma cell line A549 in vitro, and to explore the related mechanism. Methods: MTT assay was carried out to assess the viability of luteolin in A549 cell lines. The effect of luteolin on the motility of A549 cells was evaluated by wound healing assay. Focal adhesion kinase (FAK) and the phosphorylated FAK was analyzed by western blotting. Results: MTT assay showed luteolin suppressed the growth of A549 cells dose-dependently with IC₅₀ (54.4 μmol·L^-1). Our study chose 15 μmol·L^-1 luteolin to examine its effect on cell motility. The results showed that luteolin impaired the motility of A549 cells in 24 h and 48 h compared with that in 0 h. Luteolin dampened FAK phosphorylation, furthermore, total protein of FAK was also reduced moderately after treatment with luteolin. Conclusion: luteolin inhibits the motility of human lung adenocarcinoma cell line A549 in vitro, via the modulation of the key molecular of FAK.

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