目的:制备阿霉素牛血清白蛋白纳米粒(DOX-BSA-NP),观察其形态、粒径及药物缓释规律;制备并初步鉴定甲胎蛋白(AFP)单抗导向阿霉素白蛋白免疫毫微球(antiAFP- DOX-BSA-NP)。方法:以阿霉素、牛血清白蛋白为材料,采用去溶剂化法制备DOX-BSA-NP;使用电镜技术观察其形态及颗粒大小;采用胰蛋白酶消化方法测定其载药量和包封率,测定体外释药性质。采用SPDP交联法制备单抗导向的载阿霉素免疫毫微球,使用电镜技术观察其形态及颗粒大小,凝胶电泳测定共价连接。结果:(1) DOX-BSA-NP呈圆球状,外观呈红色;粒径大小均匀,载药量约为1.8%,包封率为90.3%,体外释药持续缓慢,第7天时,累积释药分数达到95%。(2) 采用SPDP交联方法制备的antiAFP-DOX-BSA-NP呈球形,粒径330~400 nm。结论:采用去溶剂化法可制备出具有缓释效果的阿霉素白蛋白微球;SPDP方法可以将单克隆抗体与载药微球连接制备出具有免疫功能的毫微球。 |
[1] RAHIMNEJAD M,JAHANSHAHI M,NAJAFPOUR G D.Production of biological nanoparticles from bovine surum albumin for drug delivery[J].Biotechnology,2006,5(20):1918-1923.
[2] FELIX K.Albumin as a drug carrier:design of prodrugs,drug conjugates and nanoparticles[J].J Control Release,2008,132:171-183.
[3] JAMES D B,BETANCOURT T,BRANNON-PEPPAS L.Active targeting schemes for nanoparticle systems in cancer therapeutics[J].Adv Drug Deliv Rev,2008,60(15):1615-1626.
[4] LI F Q,SU H,WANG J,et al.Preparation and characterization of sodium ferulate entrapped bovine serum albumin nanoparticle for liver targeting[J].Int J Pharm,2008,349(1-2):274-282.
[5] LI Q Y,LIU C,ZHANG X H,et al.Preparation,characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells[J].Int J Nanomedicine,2011,6:397-405.
[6] DAN P,KARP J M,HONG S,et al.Nanocarriers as an emerging platform for cancer therapy[J].Nat Nanotechnol,2007,2(12):751-760.
[7] CARLSSON J,DREVIN H,AXEN R.Protein thiolation and reversible protein-protein conjugation[J].Biochem J,1978,173(6):723-737.
[8] 曲秋莲,张英鸽.纳米技术和材料在医学上应用的现状与展望[J].东南大学学报:医学版,2011,30(1):157-163.
[9] HAUN J B,DEVARAJ N K,HILDERBRAND S A,et al.Bioorthogonal chemistry amplifies nanoparticle binding and enhances the sensitivity of cell detection[J].Nat Nanotechnol,2010,5(9):660-665.
[10] SHI M,LU J,SHOICHET M S.Organic nanoscale drug carriers coupled with ligands for targeted drug delivery in cancer[J].J Mater Chem,2009,19:5485-5498.
[11] BRANNON-PEPPAS L,BLANCHETTE J O.Nanoparticle and targeted systems for cancer therapy[J].Adv Drug Deliv Rev,2004,56(11):1649-1659.
[12] 王子妤,王丽,张东生.纳米雄黄脂质体的制备﹑特性检测和体外抗肿瘤细胞作用的研究[J].东南大学学报:医学版,2009,28(3):175-179.
[13] SHI M,HO K,KEATING A,et al.Doxorubicin-conjugated immuno-nanoparticles for intracellular anticancer drug delivery[J].Adv Funct Mater,2009,19:1689-1696.
[14] MAHMOUDIAN J,JEDDI-TEHRANI M,RABBANI H,et al.Conjugation of R-phycoerythrin to polyclonal antibody and F(ab')2 fragment of a polyclonal antibody by two different methods[J].Avicenna J Med Biotechnol,2010,2(2):87-91. |