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卡铂对子宫内膜癌裸鼠皮下移植瘤生长及Tie2基因表达的影响
作者:郭斐斐1  周怀君2  寻庆英1 
单位:1. 东南大学 医学院, 江苏 南京 210009;
2. 南京大学医学院附属鼓楼医院 妇产科, 江苏 南京 210008
关键词:卡铂 子宫内膜癌 裸鼠 酪氨酸激酶受体 
分类号:R737.33; R-332
出版年·卷·期(页码):2012·31·第三期(302-306)
摘要:

目的:研究卡铂对子宫内膜癌裸鼠皮下移植瘤生长的抑制作用及对肿瘤组织中酪氨酸激酶受体(Tie2)的影响。方法:应用扩增培养的Ishikawa细胞株建立子宫内膜癌裸鼠皮下移植瘤模型。随机将10只成瘤裸鼠分为卡铂化疗组(化疗组)和空白对照组(对照组),分别应用伯尔定注射液(25.0 mg·kg-1)和5%葡萄糖(0.2 ml·只-1)瘤体内注射治疗,观察移植瘤体积及瘤体质量变化,计算抑瘤率;实时定量PCR和蛋白质印迹法检测移植瘤组织中Tie2 mRNA和蛋白表达。结果:化疗组移植瘤体积为(175.99±29.57)mm3,对照组为(521.05±76.36)mm3,两组比较差异有统计学意义(P<0.05);化疗组肿瘤质量较对照组减轻(P<0.001);化疗组抑瘤率为66.22%±6.15%。化疗组Tie2 mRNA及蛋白表达水平均低于对照组(分别为P<0.05、P<0.001)。结论:卡铂具有抑制子宫内膜癌裸鼠皮下移植瘤生长的作用,肿瘤组织中Tie2的表达受到抑制,提示其在抗肿瘤过程中可能具有抗血管生成的作用。

Objective: To investigate the inhibition effect of carboplatin on the growth of xenograft in a nude mouse model of endometrial carcinoma, and the impact on the expression of tyrosine kinase receptor Tie2. Methods: The nude mouse model of endometrial carcinoma was constructed by Ishikawa cell line cultured and amplified.Ten nude mice with xenografts were randomly devided into two groups: carboplatin group and blank group.The two groups were received intratumor carboplatin(25.0 mg·kg-1)and 5% glucose(0.2 ml·mouse-1)administration respectively.The weights, sizes of the tumors and the inhibitory rates were calculated.The expression of Tie2 messenger RNA(mRNA) and protein were examined by real-time quantitative polymerase chain reaction(real-time PCR) and Western blotting respectively. Results: The average size of xenografts in the carboplatin group was (175.99±29.57) mm3, which was significantly lower than that in the blank group(P<0.05), the tumor weight in the carboplatin group was statistically significant lower than that in the blank group(P<0.001), and the inhibitory rate of the carboplatin group was 66.22%±6.15%.The differences of Tie2 mRNA and protein expression levels between the two groups were significant(P<0.05,P<0.001). Conclusion: Carboplatin can significantly inhibit the tumor growth and the expression of Tie2 in nude mice model of endometrial carcinoma, which may be related with the inhibitory effect of carboplatin on the tumor angiogenesis.

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