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干扰素对肺腺癌A549细胞B7-H4 分子表达的影响
作者:孙思庆1  姜纯国1  林勇1  金月玲2  黄培林2 
单位:1. 东南大学附属中大医院 呼吸科, 江苏 南京 210009;
2. 东南大学 医学院, 江苏 南京 210009
关键词:干扰素 肺腺癌细胞 B7-H4 细胞增殖 
分类号:R734.2; R-33
出版年·卷·期(页码):2012·31·第三期(277-281)
摘要:

目的:探讨IFN-α和IFN-γ对人肺腺癌细胞株A549 B7-H4 mRNA和蛋白表达的影响。方法:用不同浓度(100、500、1 000、2 000 U·ml-1)IFN-α和IFN-γ分别处理A549细胞,未经药物处理细胞作对照(对照组)。用MTT法观察细胞增殖活性的差异,RT-PCR观察B7-H4 mRNA表达水平的变化,蛋白质印迹法观察B7-H4蛋白表达水平的变化。结果:IFN-α和IFN-γ对人肺腺癌细胞株A549增殖有抑制作用,其抑制效应具有时间和剂量依赖性(P<0.05)。随着IFN-α浓度的增加,B7-H4分子mRNA和蛋白表达增强(P<0.05),而IFN-γ对B7-H4分子mRNA和蛋白表达影响不明显(P>0.05)。结论:IFN-α和IFN-γ均可抑制人肺腺癌A549细胞株的增殖;IFN-α可增强B7-H4mRNA和蛋白水平的表达,而IFN-γ对B7-H4分子mRNA和蛋白表达影响不明显。

Objective: To explore the effects of IFN-α and IFN-γ on B7-H4 expression in human lung adenocarcinoma cell line A549. Methods: Human lung adenocarcinoma A549 cells were treated with a concentration of 100, 500, 1 000, 2 000 U·ml-1 IFN-α and IFN-γ,respectively.The inhibitive effect of IFN-α and IFN-γ on proliferation of A549 cells was detected by MTT assay.B7-H4 mRNA expression, by retro-transcriptase polymerase chain reaction (RT-PCR); B7-H4 protein expression, by Western blotting assay. Results: IFN-α and IFN-γ inhibited the proliferation of human lung adenocarcinoma cell line A549 in a dose-, time-dependent manner(P<0.05).With the increasing in IFN-α concentration, B7-H4 molecule mRNA and protein expression increased(P<0.05).But there was no change of B7-H4 molecule mRNA and protein expression with the increasing in IFN-γ concentration. Conclusion: IFN-α and IFN-γ may inhibit the growth of human lung adenocarcinoma cell line A549,and IFN-α may enhance B7-H4 mRNA and protein expression. IFN-γ has no effect of B7-H4 mRNA and protein expression in A549.

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