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双调控溶瘤腺病毒携带SEA基因靶向鼠膀胱癌的表达
作者:陈猛1  郝林2  史振铎2  张辉1  董洋1  韩从辉2 
单位:1. 东南大学 医学院, 江苏 南京 210009;
2. 东南大学医学院附属徐州医院 泌尿外科, 江苏 徐州 221009
关键词:溶瘤腺病毒 膀胱癌 金黄色葡萄球菌肠毒素A 人端粒酶逆转录酶 缺氧诱导因子 
分类号:R737.1; R73-354
出版年·卷·期(页码):2012·31·第二期(166-169)
摘要:

目的:研究hTERT和HIF启动子双调控的溶瘤腺病毒能否感染鼠膀胱癌细胞并表达治疗基因,为动物实验提供依据。方法:以不同浓度溶瘤腺病毒感染鼠膀胱癌细胞,生物倒置显微镜下动态观察细胞形态变化,RT-PCR检测SEA在细胞内mRNA表达,Western blot检测SEA蛋白表达。结果:镜下可见细胞感染腺病毒并最终裂解,RT-PCR和Western blot分别检测到实验组mRNA和蛋白的表达。结论:携带SEA基因的hTERT/HIF双调控溶瘤腺病毒可在鼠膀胱癌细胞内复制增殖并表达SEA基因,可用于治疗鼠膀胱癌的动物实验研究。

Objective: To study human telomerase reverse transcriptase (hTERT)/hypoxia-inducible factor (HIF)-promoter regulated adenovirus whether can infect the mouse bladder cancer and express theoretical gene, providing evidence for the animal experiment. Methods: The cells of the mouse bladder cancer were infected with different concentration of adenovirus; The morphologic changes of cells were observed under the microscope; Staphylococcal enterotoxins A(SEA) in the nucleic acid expressions in tumor cells were detected by real-time quatiative polymerase chain reaction(RT-PCR) and protein expressions of SEA were detected by Western blot. Results: The cells were infected by adenovirus and disrupted, finally. The mRNA and protein of SEA were successfully expressed. Conclusion: The adenovirus can replicate and proliferate in the mouse bladder cancer cells and can be used in treating the mouse bladder cancer in vivo.

参考文献:

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