双调控溶瘤腺病毒携带SEA基因靶向鼠膀胱癌的表达-东南大学学报医学版

双调控溶瘤腺病毒携带SEA基因靶向鼠膀胱癌的表达

单位：1. 东南大学医学院, 江苏南京 210009;
2. 东南大学医学院附属徐州医院泌尿外科, 江苏徐州 221009

分类号：R737.1; R73-354

出版年·卷·期（页码）：2012·31·第二期（166-169）

摘要：

目的:研究hTERT和HIF启动子双调控的溶瘤腺病毒能否感染鼠膀胱癌细胞并表达治疗基因,为动物实验提供依据。方法:以不同浓度溶瘤腺病毒感染鼠膀胱癌细胞,生物倒置显微镜下动态观察细胞形态变化,RT-PCR检测SEA在细胞内mRNA表达,Western blot检测SEA蛋白表达。结果:镜下可见细胞感染腺病毒并最终裂解,RT-PCR和Western blot分别检测到实验组mRNA和蛋白的表达。结论:携带SEA基因的hTERT/HIF双调控溶瘤腺病毒可在鼠膀胱癌细胞内复制增殖并表达SEA基因,可用于治疗鼠膀胱癌的动物实验研究。

Objective: To study human telomerase reverse transcriptase (hTERT)/hypoxia-inducible factor (HIF)-promoter regulated adenovirus whether can infect the mouse bladder cancer and express theoretical gene, providing evidence for the animal experiment. Methods: The cells of the mouse bladder cancer were infected with different concentration of adenovirus; The morphologic changes of cells were observed under the microscope; Staphylococcal enterotoxins A(SEA) in the nucleic acid expressions in tumor cells were detected by real-time quatiative polymerase chain reaction(RT-PCR) and protein expressions of SEA were detected by Western blot. Results: The cells were infected by adenovirus and disrupted, finally. The mRNA and protein of SEA were successfully expressed. Conclusion: The adenovirus can replicate and proliferate in the mouse bladder cancer cells and can be used in treating the mouse bladder cancer in vivo.

参考文献：

[1] 胡建鹏,郝林,张培影,等.双调控溶瘤腺病毒介导超抗原SEA基因靶向膀胱癌肿瘤表达[J].中华实验外科杂志,2010,27(8):1131-1133.
[2] SHIEH G S,SHIAU A L,YO Y T,et al.Low-dose etoposide enhances telomerase-dependent adenovirus-mediated cytosine deaminase therapy through augmentation of adenoviral infection and transgene expression in a syngeneic bladder tumor model[J].Cancer Res,2006,66(20):9957-9966.
[3] 杨立新,张海峰,白淑芬.P53、Ki-67基因表达与膀胱癌相关性的回顾性研究[J].现代医学,2010,38(1):32-35.
[4] 应文群,李巧星.hTERT和survivin在膀胱癌移行细胞癌中的表达及意义[J].肿瘤基础与临床,2006,19(1):19-20.
[5] 何汀,陈明,章宜芬,等.Mel-18在人膀胱尿路上皮肿瘤组织中的表达及意义[J].现代医学,2010,38(3):212-216.
[6] 陈波,贺厚光,韩从辉,等.二次经尿道膀胱肿瘤电切治疗非浸润性膀胱肿瘤[J].东南大学学报:医学版,2010,29(6):665-667.
[7] MORALES A,EIDENGER D,WARD A W.Intracavity Bacillus Calmette-Guerin in the treatment of superficial bladder tumours[J].J Urol,1976,116(2):180-183.
[8] GU J,ANDREEFF M,ROTH J A,et al.hTERT promoter induces tumor-specific Bax gene expression and cell killing in syngenic mouse tumor model and prevents systemic toxicity[J].Gene Ther,2002,9(1):30-37.
[9] 龙跃生,赵绮华,曾涛,等.人及小鼠钠通道SCN3A基因的启动子及其上游调控区的分析[J].生物化学与生物物理进展,2009,36(3):339-345.

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