近红外光谱技术在激光热疗荷瘤大鼠中的实时监测研究-东南大学学报医学版

近红外光谱技术在激光热疗荷瘤大鼠中的实时监测研究

单位：1. 东南大学附属中大医院神经外科, 江苏南京 210009;
2. 南京航空航天大学生物医学工程系, 江苏南京 210016

分类号：R73-354; R730.57; R730.264

出版年·卷·期（页码）：2012·31·第二期（131-135）

摘要：

目的:近红外光谱(NIRS)技术应用于激光间质热疗大鼠胶质瘤术中实时监测,观察术中NIRS参数变化规律,探讨利用NIRS对胶质瘤激光间质热疗进行实时监测的可行性。方法:将传代培养的C6胶质瘤细胞接种于大鼠左前腋皮下,建立荷瘤大鼠模型,肿瘤生长至直径1~1.5cm时,在不同时间、功率下对大鼠荷瘤进行808nm激光间质热疗,观察并记录激光间质热疗时瘤组织的NIRS参数,尤其是优化散射系数(μ's)的变化情况。结果:不同时间、功率下NIRS变化显著。结论:利用NIRS实时活体在位监测激光间质热疗大鼠胶质瘤是可行的。

Objective: To explore the feasibility of using near-infrared spectroscopy (NIRS) technology to monitor real-time in laser interstitial thermotherapy for rats with glioma and to observe the process of changes in the optical parameters. Methods: Injecting C6 glioma cells into the left upper limb subcutaneous tissue of rats to establish animal models, when tumor become 1-1.5 cm; Using 808 nm laser irradiate glioma with NIRS monitoring in different time and power; the changes of optical parameters, the reduced scattering coefficient in particular, were observed and recorded by glioma tissue spectroscopy. Results: NIRS changed evidently under different time and power. Conclusion: Real-time use of NIRS technology in laser interstitial thermotherapy for rats with glioma is feasible.

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