伽马刀放疗腹膜后淋巴结转移癌对肠道菌群的影响-东南大学学报医学版

伽马刀放疗腹膜后淋巴结转移癌对肠道菌群的影响

单位：1. 第二军医大学海军临床学院,北京 100048;
2. 中国人民解放军海军总医院消化内科,北京 100048;
3. 中国人民解放军海军总医院放疗科,北京 100048;
4. 中国人民解放军海军总医院中心实验室,北京 100048

关键词：腹膜后淋巴结转移癌 γ刀放疗肠道菌群实时荧光定量聚合酶链式反应

分类号：R-33; R815.2

出版年·卷·期（页码）：2012·31·第一期（72-78）

摘要：

目的:观察腹膜后淋巴结转移癌γ刀放疗后肠道菌群的变化。方法:选取经临床表现及影像学、病理学确诊的腹膜后淋巴结转移癌并拟行γ刀放疗患者40例为观察组,健康志愿者20例为正常对照组,采用实时荧光定量聚合酶链式反应(PCR)检测观察组及正常对照组粪便中大肠杆菌、肠球菌属、双歧杆菌属及乳酸杆菌属细菌数量,并对两组结果进行比较分析。结果:与正常对照组比较,观察组患者γ刀放疗前及放疗后1周时粪便中4种目标菌群细菌数量无明显变化(P>0.05);γ刀放疗1个月后,粪便中大肠杆菌及肠球菌属细菌数量仍无显著变化(P>0.05),但双歧杆菌属及乳酸杆菌属细菌数量明显减少(P<0.05)。结论:使用γ刀放疗腹膜后淋巴结转移癌1个月不会引起大肠杆菌及肠球菌属细菌数量的改变,但可导致双歧杆菌属及乳酸杆菌属细菌数量明显下降。

Objective: To observe the change of intestinal microflora after γ knife radiotherapy in patients with retroperitoneal lymph node metastases. Methods: In this test, 40 patients with retroperitoneal lymph node metastases were selected as observation group, they were diagnosised by clinical manifestations, imaging and pathologic findings, all these cases were given γ knife radiotherapy. Tweenty healthy volunteers were selected as the control group. The number of E.coli, Enterococcus, Bifidobacterium and Lactobacillus in early morning stool of these patients were detected before γ knife radiotherapy, 1 week after radiotherapy, 1 month after radiotherapy, and the healthy control group by real-time fluorescence quantitative PCR, and every target bacteria was analysized. Results: Compared with the control group, the 4 kinds of target bacteria in the facels of the patients before γ knife radiotherapy and 1 week after radiotherapy had no significant change(P>0.05). One month after radiotherapy, the number of E.coli and Enterococcus still had no significant change(P>0.05), but the number of Bifidobacterium and Lactobacillus decreased significantly(P<0.05). Conclusions: Using γ knife radiotherapy to therapy retroperitoneal lymph node metastases couldn't cause the number of E.coli and Enterococcus changes in a month, but it could cause the number of intestinal Bifidobacterium and Lactobacillus decrease significantly.

参考文献：

[1] RINTTILA T,KASSINEN A,MALINEN E,et al.Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by samples by real-time PCR[J].J Appl Microbiol,2004,97(6):1166-1177.
[2] 韩晓云,邓红,蔡燕.肠道微生物与慢性病[J].中国微生态杂志,2009,11(21):1039-1042.
[3] SGHIR A,GRAMET G,SUAU A,et al.Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization[J].Appl Environ Microbiol,2000,66(5):2263-2266.
[4] HILL M J,DRASAR B S.The normal colonic bacterial flora[J].Gut,1975,16(4):318-323.
[5] 朱捷,杨成君,王军.荧光定量PCR技术及其在科研中的应用[J].生物技术通报,2009,(2):73-76.
[6] WONG M L,MEDRANO J F.Real-time PCR for mRNA quantitation[J].Biotechniques,2005,39(1):75-85.
[7] GALBIATI S,SMID M,GAMBINI D,et al.Fetal DNA detection in material plasma throughout gestation[J].Hum Genet,2005,177(2-3):243-248.
[8] 彭波,陈思玮.放射性肠炎患者肠道菌群的变化及双歧三联活菌制剂的疗效研究[J].中国现代医学杂志,2000,10(10):85-86.
[9] 管远志.肠道菌群及其生物学意义[J].临床儿科杂志,2009,27(11):1095-1097.
[10] NOBAEK S,JOHANSSON M L,MOLIN G,et al.Alteration of intestinal microflora is associated with reduction in abdominal bloating and pain in patients with irritable bowel syndrome[J].Am J Gastroenterol,2000,95(5):1231-1238.
[11] ALEXANDER J W,GIANOTTI L,PYLES T,et al.New insights into the pathogenesis of radiation-induced intestinal dysfunction[J].Aliment Pharmacol Ther,2000,14(5):523-528.
[12] KAO M S.Intestinal complications of radiotherapy in gynecologic malignancy-clinical presentation and management[J].Int J Gynecol Obstet,1995,49(Suppl):S69-75.

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