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黄芪甲苷的抗氧化性对缺氧缺糖 H9c2细胞的保护作用探讨
作者:承燕  吕磊  江时森 
单位:南京大学医学院临床学院,南京军区南京总医院 心内科,江苏 南京 210002
关键词:黄芪甲苷 H9c2细胞 缺氧 自由基 
分类号:R-33; R285.5
出版年·卷·期(页码):2012·31·第一期(51-55)
摘要:

目的:探讨黄芪甲苷(AST-Ⅳ)的抗氧化性对缺氧缺糖H9c2细胞的保护作用。方法:对用无糖Na2S2O4溶液制造的细胞缺氧模型给予AST-Ⅳ预处理,同时以抗氧化能力相近的抗氧化剂水溶性维生素E(Trolox)作为对照检测细胞内氧自由基(ROS)、丙二醛(MDA)的浓度和乳酸脱氢酶(LDH)漏出率,CCK-8检测细胞存活率,FITC-annexin V/PI双染检测凋亡情况。结果:经60 μmol·L-1的AST-Ⅳ预处理后,细胞内ROS、MDA浓度较模型组有明显下降(P<0.01),下降程度与Trolox预处理组相近。AST-Ⅳ预处理组细胞存活率明显高于模型组(P<0.01),但低于Trolox预处理组(P<0.01)。AST-Ⅳ预处理组LDH漏出率和细胞凋亡率较模型组均下降(P<0.01),但不如Trolox预处理组下降明显(P<0.01)。结论:AST-Ⅳ能减轻无糖Na2S2O4溶液造成的细胞氧化应激,但效果不如Trolox,其抗氧化性对H9c2细胞的保护作用有限。

Objective: To investigate the protection effect of antioxidant activity of astragaloside Ⅳ(AST-Ⅳ) on H9c2 cells injured by hypoxia. Methods: AST-Ⅳ was administrated an hour before oxygen and glucose deprivation, and the levels of intracellular ROS and malondialdehyde(MDA), cell viability, apoptosis and necrosis were measured and were compared with Trolox which had an equal antioxidant capacity. Cell viability was determined by a CCK-8 kit. Cell necrosis was assayed by the ratio of released LDH. FITC-annexin V/PI was used for apoptosis. Results: The intracellular ROS and MDA formation after 60 μmol·L-1 AST-Ⅳ pretreatment decreased as low as after Trolox pretreatment. The cell viability, ratio of released LDH and apoptosis were ameliorated in AST-Ⅳ pretreatment group compared to hypoxia group(P<0.01). However, Tolox pretreatment improved those indexes better than AST-Ⅳ pretreatment(P<0.01). Conclusion: AST-Ⅳ reduces the oxidative stress in H9c2 cells injured by oxygen and glucose deprivation, while it is less effective than Trolox. The antioxidant properties of AST-Ⅳ has limitations in protective effect.

参考文献:

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