RP-HPLC定量三乙胺法测定中药黄连中盐酸小檗碱的含量-东南大学学报医学版

RP-HPLC定量三乙胺法测定中药黄连中盐酸小檗碱的含量

单位：1. 广东医学院生物化学与分子生物学教研室,广东东莞 523808;
2. 广东医学院医学活性分子研究重点实验室,广东东莞 523808;
3. 广东医学院中药新药研究所,广东东莞 523808

分类号：R-33; R917

出版年·卷·期（页码）：2012·31·第一期（8-11）

摘要：

目的:建立一种反向高效液相色谱(RP-HPLC)法测定黄连中盐酸小檗碱的含量。方法:采用HPLC法,色谱柱为Luna C18柱(150 mm×4.60 mm,5 μm),流动相为乙腈-0.1%磷酸(25 ∶75),每1 000 ml 0.1%的磷酸溶液中加入50 μl的三乙胺(扫尾剂)进行洗脱;检测波长为340 nm。结果:采用HPLC法检测黄连中盐酸小檗碱的含量,其在0.246~2.460 μg范围内与峰面积积分值呈良好的线性关系。平均加样回收率为100.1%,相对标准偏差(RSD)为1.60%。结论:HPLC简便可行、重复性好,可作为检测盐酸小檗碱含量的质量控制;一定量的三乙胺分析纯可作为很好的扫尾剂,用于盐酸小檗碱的含量测定。

Objective: To establish a new RP-high-performance liquid chromatography(HPLC) method for detecting the content of Berberine hydrochloride(BH) in coptis chinensis franch. Methods: The content of BH in coptis chinensis franch was deteminated by HPLC. HPLC conditions for the column: Luna C18(150 mm×4.60 mm, 5 μm), acetonitrile-0.1% phosphoric acid (25 ∶75), and adding 50 μl Triethylamine(tailing-suppressing reagent) into every 1 000 ml 0.1% phosphoric acid for elution, the detection wavelength was 340 nm. Results: This HPLC method exhibited a linear relationship between the content of BH within 0.246 to 2.460 μg and the peak area integration, the average recovery rate of BH was 100.1%, the relative standard deviation(RSD) was 1.60%. Conclusion: This is a simple, reproducible and reliable HPLC method for determinating the content of BH. A certain amount of Triethylamine analytically pure reagent is a very good tailing-suppressing reagent for using HPLC method to determinate the content of BH.

参考文献：

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