Objective To investigate whether or not that the four kind of components pured from conspecific inner ear membranous labyrinth tissue immunize guinea pig and induce experimental autoimmune Meniere's disease.At the same time,we would like to determine these antigen's distributing and expressing position in inner ear.Methods 70 guinea pigs were respectively divided into group B,group C,group D and group E and were immunized respectively with components of inner ear tissue antigen (28 000,42 000,58 000 and 68 000 proteins).Auditory brainstem response(ABR),level of specific antibody against every component of inner ear antigen levels in serum,vestibular function and pathohistological morphological changes of inner ear were observed.The distribution of protein antigen and expressing position in inner ear were located with immunohistochemistry observation.Results After immunization,there were no significant changes of hearing threshold in group A and group B,also no inner ear morphologic changes were found in A and B group.The levels of specific antibodies in serum of group B,C,D and E groups increased significantly after immunization.No animals were found to have hearing loss after immunization in group B,3 animals (4 ears) in group C,4 animals (6 ears) in group D and 4 animals (6 ears) in group E were found to have hearing loss.Labyrinthine hydrops and decrease of spiral ganglion cells were found in some animal ears of group C,D and E,all these ears were found to have hearing loss.The 68 000 protein mainly distributed at stria vascularis and small the Corti's organ;The 28 000 distributed strictly in the spiral ganglion;The 42 000 and 58 000 protein widely distributed in membranous labyrinth,including the spiral ganglion,Corti's organ,stria vascularis and spiral ligament.Conclusion Three components of the inner ear tissue antigen,the 42 000,58 000 and 68 000 could respectively induce autoimmune Meniere's disease,which means more than one components of inner ear antigens are responsible for the experimental AIMD.