Objective To analyze the distribution of clinical encountered pathogens and their drug resistance,and to understand epidemic situation of causative agent and provide reference for reasonable choices of the clinical antibiotics.Methods Identification and bacteria-susceptibility test were operated with BioFoSun Microbiology Identification and Antimicrobial Susceptibility Test System.Results Totally 832 strains of pathogenic bacteria were isolated,they comprised 178 strains of Gram-positive bacteria,563 strains of Gram-negative bacteria and 91 strains of fungi.The main Gram-positive microorganisms included staphylococcus aureus. The main Gram-negative microorganisms included Escherichia coli,Klebsiella,spp,Acinetobacter sp,Pseudomonas aeruginosa,The most resistant pathogen was Escherichia coli, which was found resistant to most of antimicrobial agents.No strains of Staphylococcus and Enterococcus were found resistant to vancomycin.Meropenem and imipenem were the most potent antimicrobial agents,the second was amikacin.Conclusion In the pathogenic bacteria in nosocomial infection of our prefecture, Escherichia coli were found resistant to most of antimicrobial agents.It should be thought highly by clinic.