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血管内皮生长因子165基因对人胃癌细胞侵袭性的影响
作者:乐红琴1 2  欧希龙2  杭程2  陈慧娟2 
单位:1. 东南大学医学院附属盐城医院 消化内科, 江苏 盐城 224001;
2. 东南大学附属中大医院 消化内科, 江苏 南京 210009
关键词:血管内皮生长因子165 胃癌 侵袭 迁移实验 基质金属蛋白酶-2 尿激酶型纤溶酶原激活物 
分类号:R735.2; Q786
出版年·卷·期(页码):2009·28·第二期(118-122)
摘要:

目的: 探讨血管内皮生长因子165(VEGF165)基因对胃癌细胞侵袭性的影响及可能机制。方法: 通过体外培养人胃腺癌细胞BGC-823,观察胃癌细胞转染VEGF165基因对其迁移能力及侵袭相关分子MMP-2、u-PA mRNA表达的影响。应用Millicell小室分析VEGF165转染前后胃癌细胞迁移能力的变化;逆转录聚合酶链反应(RT-PCR)研究VEGF165转染前后BGC-823的 MMP-2、u-PA mRNA表达变化。结果: 迁移实验结果显示Ad-VEGF165组胃癌细胞迁移能力高于Ad-GFP组及对照组 10.148 8) vs (142.666 7±14.502 8) and (148.333 3±7.571 8),P <0.05];RT-PCR结果显示VEGF165转染后促进了BGC-823 的MMP-2、u-PA mRNA表达,Ad-VEGF165组MMP-2、u-PA的mRNA水平明显高于Ad-GFP组及对照组-2 mRNA:(0.664 6± 0.048 6)vs (0.409 6±0.066 8) and (0.380 3±0.025 4);u-PA mRNA:(0.821 6±0.079 8) vs (0.406 0±0.115 8) and (0.404 3±0.062 0);均为P <0.05]。结论: 经VEGF165转染后,胃癌细胞迁移能力增强,MMP-2、u-PA的mRNA表达增加,提示VEGF在肿瘤的侵袭过程中发挥促进作用,MMP-2、u-PA可能与此作用有关。

Objective To study the effects of vascular endothelial growth factor 165 (VEGF165) on the invasion of human gastric cancer cells and it's underlying mechanism.Methods Human gastric cancer cell line BGC-823 was used in this study.BGC-823 was transfected with Ad-GFP and Ad-VEGF165.A Millicell cell culture chamber assay in vitro was used to detect the ability of migration of BGC-823.The influence of VEGF165 on the mRNA expression of matrix metalloproteinases-2 (MMP-2) and urokinase-type plasminogen activator(u-PA) was investigated by reverse transcription polymerase chain reaction (RT-PCR).Results Migration assay showed that the migration rate of BGC-823 was significantly higher in the Ad-VEGF165 group than that in the Ad-GFP and control groups [212.0000±10.148 8 vs (142.666 7± 14.502 8) and (148.333 3±7.571 8),P <0.05].RT-PCR displayed that the VEGF165-transfected BGC-823 increased the expression of MMP-2 and u-PA mRNA,which was higher in the Ad-VEGF165 group than in the Ad-GFP group and control group [MMP-2 mRNA:(0.664 6 ±0.048 6) vs (0.409 6 ±0.066 8) and (0.380 3±0.025 4);u-PA mRNA:(0.821 6±0.079 8) vs (0.406 0±0.115 8) and (0.404 3 ±0.062 0);P <0.05].Conclusions VEGF165-transfected can enhance the migration ability of human gastric cancer cell BGC-823,the expression of MMP-2 and u-PA mRNA was higher in the Ad-VEGF165 group than in the Ad-GFP group and control group.Which prompt VEGF in the invasion of tumor plays a catalytic role.This effect may be related to the up-regulated expression of MMP-2 and u-PA.

参考文献:

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