超声联合微泡对内皮祖细胞体外增殖、凋亡及细胞周期的影响-东南大学学报医学版

超声联合微泡对内皮祖细胞体外增殖、凋亡及细胞周期的影响

单位：1. 东南大学临床医学院, 江苏南京 210009;
2. 东南大学生物科学与医学工程学院, 江苏南京 210096

分类号：R454.3; Q813

出版年·卷·期（页码）：2009·28·第二期（109-112）

摘要：

目的: 体外探讨超声联合微泡对内皮祖细胞增殖、凋亡及细胞周期的影响,为进一步体内研究提供科学依据。方法: 体外提取、分离和培养猪骨髓内皮祖细胞,随机分为对照组、超声组、超声微泡组,干预后24 h分别采用CCK-8检测细胞增殖活性,PI染色流式细胞仪分析细胞凋亡坏死及细胞周期。结果: (1)低声强超声(0.25~1.0 W·cm^-2)联合微泡作用下对内皮祖细胞增殖活性无明显影响;较高声强(﹥1.0 W·cm^-2)作用下,随声强逐渐增大,细胞增殖活性逐渐降低。(2)与对照组比较,超声微泡组(频率1 MHz,输出功率1.0 W·cm^-2,辐照90 s)对内皮祖细胞凋亡坏死及细胞周期无明显影响,而同样条件下超声组细胞凋亡坏死明显增加,同时显著抑制细胞DNA合成。结论: 1.0 W·cm^-2超声联合微泡干预内皮祖细胞对细胞增殖活性、凋亡坏死及细胞周期无明显不良影响,为进一步应用超声微泡体内干预内皮祖细胞移植的研究提供可能。

Objective To investigate the effect of ultrasound-mediated microbubble destruction on the proliferation,apoptosis and cell cycle of endothelial progenitor cells (EPCs)ex vivo.Methods Bone marrow-derived EPCs of Chinese minipigs were collected and cultured in vitro.The EPCs were divided into 3 groups:control group,ultrasound group and ultrasound-mediated microbubble group.Three groups EPCs suffered from intervene and after 24 h apoptosis and cell cycle were observed by PI labeled staining and flow cytometric assay respectively.Results (1) Ultrasound-mediated microbubble destruction in the low sound intensity(0.25—1.0 W·cm^-2)had no effect on proliferation of EPCs;but as sound intensity was increased higher than 1.0 W·cm^-2,the extent of EPCs proliferation was decreased.(2) Compared to control group,ultrasound-mediated microbubble destruction in 1.0 W·cm^-2 had no effect on apoptosis/necrosis and cell cycle of EPCs,inversely ultrasound remarkably accelerated the apoptosis/necrosis and inhibited DNA synthesis of EPCs.Conclusion 1.0 W·cm^-2 ultrasound-mediated microbubble destruction has no adverse effect on proliferation,apoptosis/necrosis and cell cycle of endothelial progenitor cells ex vivo.

参考文献：

[1] ALAN D L,COLIIN D M,MAJID E,et al.Global and regional burden of disease and risk factors,2001:systematic analysis of population health data[J].Lancet,2006,367:1747-1757.
[2] VINCENT F M S,RICHARD T L.Stem-cell therapy for cardiac disease[J].Nature,2008,451:937-942.
[3] JI S,MING Q,SANJIV K,et al.Stimulation of arteriogenesis in skeletal muscle by microbubble destruction with ultrasound[J].Circulation,2002,106:1550-1555.
[4] SONG J I,PATRICK S C,ALEXANDER L,et al.Microvascular remodeling and accelerated hyperemia blood flow restoration in arterially occluded skeletal muscle exposed to ultrasonic microbubble destruction[J].Am J Physiol Heart Circ Physiol,2004,287(6):H2754-2761.
[5] JUNJI Y,KOJI O,HIROTO T,et al.Treatment of ischemic limbs based on local recruitment of vascular endothelial growth factor-producing inflammatory cells with ultrasonic microbubble destruction[J].J Am Coll Cardiol,2005,46:899-905.
[6] SOPHIE H,ALEXANDER L,KLIBANOV S.Microbubbles in ultrasound-triggered drug and gene delivery[J].Advanced Drug Delivery Reviews,2008,60:1153-1166.
[7] FERIL L B,KONDO T,ZHAO Q L,et al.Enhancement of hyperthermial induced apoptosis by non-thermal effects of ultrasound[J].Cancer Lett,2002,178 (1):63-70.
[8] BORIS K,EITAN K.Shear stress induced by a gas bubble pulsating in an ultrasonic field near a wall[J].IEEE Transactions on Ultrasonics,Ferroelectrics,and Frequency Control,2004,51:973-979.
[9] KIMIKO Y,TAKAAKI S,TETSURO W,et al.Fluid shear stress induces differentiation of Flk-1-positive embryonic stem cells into vascular endothelial cells in vitro[J].Am J Physiol Heart Circ Physiol,2005,288:H1915-H1924.
[10] TAO J,YANG Z,WANG J M,et al.Effects of fluid shear stress on eNOS mRNA expression and NO production in human endothelial progenitor cells[J].Cardiology,2006,106(2):82-88.
[11] TAO J,YANG Z,WANG J M,et al.Shear stress increases Cu/Zn SOD activity and mRNA expression in human endothelial progenitor cells[J].J Hum Hypertens,2007,21(5):353-358.

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