BY-1抗真菌、抗炎及镇咳祛痰作用的药效学研究-东南大学学报医学版

BY-1抗真菌、抗炎及镇咳祛痰作用的药效学研究

单位：南京工业大学, 江苏省药物研究所, 江苏南京 210009

分类号：R978.5; R974; R285.5

出版年·卷·期（页码）：2009·28·第二期（96-101）

摘要：

目的: 探讨BY-1药物的抗真菌、抗炎和镇咳祛痰作用。方法: 采用交链链格孢标准株,通过二倍稀释法,测定BY-1两种药用成分桉叶油与山苍子油不同体积比对应的最小抑菌浓度,以确定桉叶油与山苍子油的最佳配比;通过巴豆油致小鼠耳廓肿胀法及大鼠巴豆油气囊法评价BY-1的抗炎作用;采用小鼠氨水引咳法和气管酚红排泄法研究BY-1的镇咳和祛痰作用;同时与阳性对照药吉诺通进行比较。结果: 桉叶油与山苍子油1:1配比所对应的组方抗真菌效果最好,效果显著优于吉诺通。BY-1可显著减轻小鼠耳廓肿胀(P <0.05、0.01、0.001),显著减少大鼠巴豆油气囊肿的渗出液量(P <0.05),并能够明显延长小鼠的咳嗽潜伏期(P <0.05、0.01),增加气道痰液的排出量(P <0.05、0.01)。结论: BY-1具有良好的抗真菌、抗炎和镇咳祛痰作用。

Objective To investigate the antifungal activity and pharmacodynamical effects of BY-1 on anti-inflammation,relieving cough and expelling phlegm in vitro.Methods Adopting double dilution method to test minimum inhibitory concentrations of eucalyptus oil and fructus litsease oil with different volume proportion on type culture strain Alternaria alternata in order to confirm the best proportion between eucalyptus oil and fructus litsease oil; To evaluate BY-1 anti-inflammatory effect through animal experimental models,such as the swelling of ear induced by oleum crotonis in mice and rat's granulation induced by oleum crotonis;To observe the effects of relieving cough and expelling phlegm of BY-1 through ammonia water inducing cough and phenol red apophlegmating in mice.Results With proportion 1:1,the antifungal effect of eucalyptus oil and fructus litsease oil was strongest.BY-1could remarkbly inhibit the mice ear swelling(P <0.05,0.01,0.001) and reduce exudates of air cyst on experiment rats(P <0.05).In addition,the cough latency phase was obviously prolonged in BY-1 group(P <0.05,0.01),and the discharge of phenol red from mice respiratory tract was increased in BY-1 group(P <0.05,0.01).Conclusion BY-1 has significant functions of antifungi, anti-inflammation,relieving cough and expelling phlegm.

参考文献：

[1] 付文卫,赵春杰,窦德强,等.标准桃金娘油药理及临床研究进展[J].中成药,2003,25(12):1009-1011.
[2] XU S Y,BIAN R L,CHEN X.Methodology of pharmacological experiments[M].2rd ed.Beijing:Peoples Medical Publishing House,1991:719.
[3] H.沃格尔.药理学实验指南[M].杜冠华,李学军,张永祥,等译.北京:科学出版社,2001:545.
[4] 李仪奎.中药药理学实验方法[M].上海:上海科学技术出版社,1991:424-430.
[5] KALINER M A,OSGUTHORPE J D,FIREMAN P,et al .Sinusitis:bench to bedside,current findings,future directions[J].J Allergy Clin Immunol,1997,99(6 pt 3):S829-848.
[6] MANNING S C,HOLMAN M.Further evidence for allergic pathophysiology in allergic fungal sinusitis [J].Laryngoscope,1998,108(10):1485-1496.
[7] ADHIKARI A,SEN M M,CHANDA S,et al .Incidence of allergenically significant fungal aerosol in a rural bakery of West Bengal,India[J].Mycopathologia,2000,149(1):35-44.
[8] ERBEK S S,SEREFHANOGLU K,ERBEK S.Clinical subgroups and antifungal susceptibilities in fungal culture-positive patients with chronic rhinosinusitis .Eur Arch Otorhinolaryngol,2007-06-12].http://springer.lib.tsinghua.edu.cn/content/c573273686u 1244r/fulltext.html
[9] VIRANT F S.Sinusitis and asthma:associated airway diseases[J].Curr Allergy Asthma Rep,2001,1(3):277-281.
[10] LEYNAERT B,NEUKIRCH C,KONY S,et al .Association between asthma and rhinitis according to atopic sensitization in a population-based study[J].J Allergy Clin Immunol,2004,113 (1):86-93.
[11] 周汉良,陈季强.呼吸药理学与治疗学[M].北京:人民卫生出版社,1999:429.
[12] PEANA A T,D'AQUILA P S,CHESSA M L,et al .(-)-linalool produces antinociception in two experimental models of pain[J].Eur J of Pharmacol,2003,460(1):37-41.
[13] SANTOS F A,RAO V S.Antiinflammatory and antinociceptive effects of 1,8-cineole a terpenoid oxide present in many plant essential oils[J].Phytother Res,2000,14(4):240-244.
[14] KEHRL W,SONNEMANN U,DETHLEFSEN U.Therapy for acute nonpurulent rhinosinusitis with cineole:results of a double-blinded,randomized,placebo-controlled trial[J].Laryngoscope,2004,114(4):738-742.
[15] TROMBETTA D,CASTELLI F,SARPIETRO M G,et al.Mechanisms of antibacterial action of three monoterpenes[J].Antimicrob Agents And Chemother,2005,49(6):2474-2478.
[16] HAMMER K A,CARSON C F,RILEY T V.Antifungal activity of the components of Melaleuca alternifolia (tea tree) oil[J].J Appl Microbiol,2003,95:853-860.
[17] 方芳,吕昭平,王正文,等.山苍子油抗念珠菌的敏感性及作用机理的电镜研究[J].中华皮肤科杂志,2002,35(5):349-351.
[18] PARVEEN M,HASAN M K,TAKAHASHI J,et al.Response of Saccharomyces cerevisiae to a monoterpene:evaluation of antifungal potential by DNA microarray analysis[J].J Antimicrob Chemother,2004,54(1):46-55.
[19] 单秀娟,万力,董晓娜.山苍子油抗真菌的研究进展[J].中国真菌学杂志,2007,2(4):253-256.
[20] 罗曼,邹国林,蒋立科.柠檬醛抑制黄曲霉生长相关机理的研究[J].武汉大学学报:理学版,2001,47(6):745-751.
[21] 罗曼,蒋立科,邹国林.柠檬醛对黄曲霉质膜物理损伤机制的研究[J].武汉大学学报:理学版,2002,48(2):217-222.
[22] 殷志勇,王秋娟,贾莹.山苍子水提物柠檬醛抗哮喘作用的实验研究[J].中国临床药理学与治疗学,2006,11(2):197-201.
[23] 吴正三,蒋立科,陈发扬,等.山苍籽油抗霉作用研究[J].应用与环境生物学报,1997,3(2):148-153.

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