HBV感染胎盘中IL-6mRNA的表达及作用-东南大学学报医学版

HBV感染胎盘中IL-6mRNA的表达及作用

单位：1. 东南大学临床医学院, 江苏南京 210009;
2. 东南大学附属南京第二医院妇产科, 江苏南京 210003;
3. 东南大学附属南京第二医院检验科, 江苏南京 210009

分类号：R512.62

出版年·卷·期（页码）：2009·28·第一期（39-42）

摘要：

目的: 探讨白细胞介素6(IL-6)mRNA在HBV感染胎盘中的表达情况。方法: 临床上选取HBsAg(+)且HBV DNA(1~3)×10⁸copies·ml^-1孕妇60例,应用免疫组化SP方法检测其胎盘上HBsAg、HBcAg,根据HBsAg和(或)HBcAg是否表达分为胎盘感染组及非感染组,应用ELISA及RT-PCR方法检测两组孕妇分娩前血清IL-6水平及分娩时胎盘IL-6 mRNA表达。结果: 胎盘感染组42例,非感染组18例。胎盘感染组宫内感染率15/42(35.7%),非感染组宫内感染率1/18(5.56%),两组之间有显著性差异(χ²=4.38,P<0.05)。胎盘感染组胎盘局部IL-6 mRNA表达明显高于非感染组,差异有统计学意义(P<0.05)。两组血清IL-6水平无统计学差异(P>0.05)。结论: 胎盘感染是HBV宫内感染的危险因素,胎盘局部IL-6表达与胎盘HBV感染可能有一定的相关性。

Objective To investigate the expression of IL-6 mRNA in HBV placental infection.Methods 60 HBsAg-positive pregnancy women with HBV DNA(1-3)×10⁸copies·ml^-1 were enrolled,their HBsAg and HBcAg in placenta collected from those women was detected by SP immunohistochemical staining.They were divided into placental infection group and non-infection group according to positive or negative HBsAg and (or) HBcAg expression in placenta.Serum IL-6 gathered from two groups women at antepartum were determined by ELISA.Placental IL-6 mRNA was tested by RT-PCR.Results 42 cases had placental infection,18 did not.The ratio of newborn HBV intrauterine infection was 15/42(35.7%) in placental infection group,which was higher than 1/18(5.56%) in non-placental-infection group (χ²=4.38,P<0.05).Serum IL-6 was not different between two group (P>0.05).The expression of IL-6 mRNA in placental infection group was obviously higher than that of placental non-infection group (P<0.05).Conclusion Placental infection was a risk factor in HBV intrauterine infection.The expression of IL-6 mRNA in placenta was probably related to HBV placental infection.

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