Objective: To evaluate the efficacy of electromyographic biofeedback on irritable bowel syndrome(IBS). Methods: A total of 60 patients with IBS were enrolled and randomized into trial group and control group. Similar dosage of gastrointestinal prokinetie agent, microecological modulator of intestinal flora and modulator of autonomic nerve were administered as baseline therapy in both groups. The patients in trial group were treated with electromyographic biofeedback therapy for 4 weeks. Self-rating depression scale(SDS), self-rating anxiety scale(SAS), gastrointestinal symptoms rating scale(GSRS) and the threshold of visceralgia were evaluated before and after treatment. Results: After treatment, compared with the control group, the scores of SDS, SAS and GSRS in trial group declined significantly (P<0.05); the threshold of visceralgia increased significantly (P<0.05). Conclusion: Electromyographic biofeedback therapy can improve in psychological disorder, visceralgia hypersensitivity and gastrointestinal symptoms on IBS.