【Abstract】 Objective: In order to diagnose non-gonococcal urethritis / cervicitis (referred to as NGU) with mycoplasma infections for clinicians scientificly and provide the basis for rational drug use, author investigated epidemiological status and the analysis of drug sensitivity test for genital mycoplasma of STD outpatients in Nanjing. Methods:Genitourinary tract secretion of 1178 cases who were suspected patients with NGU of STD outpatients in Nanjing from January 2008 to December 2010 was tested by Ureaplasma urealyticum and Mycoplasma hominis culture, counting, drug sensitivity reagent box testing. Results: Mycoplasm culturing result show: among the 1178 cases,Ureaplasma urealyticum(Uu) positive was detected in 529 cases, with a infection rate reaching 44.9%, mycoplasma hominis (Mh) positive was detected in 190 cases, with a infection rate reaching 16.1%, Uu + Mh positive was detected in 174 cases, with a infection rate reaching 14.8%; Age distribution on 20-40 sexually active crowd incidence highest, around 70.3% ; the sex ratio (male:female)is 1:2. Drug sensitivity test result show: The highest sensitivity of mycoplasma to eight kinds of antibiotics is minocycline (MIN),secondly is doxycycline (DOX), thirdly is josamycine (JOS). The highest resistance of mycoplasma is ofloxacin (OFL), secondly is roxithromycin (ROX). Conclusions: Genitourinary tract pathogen of STD outpatients with mycoplasma infections is mainly Uu, higher in women than men,the majority of 20-40 year-old sexually active population. Drug test result show that minocycline (MIN),doxycycline (DOX) can be regarded as firstchoice for the treatment of mycoplasma infection. Paying attention to NGU pathogen detection and drug sensitivity test is rather important for doctor diagnosis scientificly and rational drug treatment, utmost ground control of drug resitant inclinical guidelines is extremely important.