Vasostatin is one of angiogenesis inhibitors which have been studied intensively because of its clinical application potential. When vasostatin protein was produced in E.coli, it expressed in insoluble and inactive inclusion bodies form. For efficient production of soluble and biologically active recombinant vasostatin, vasostatin gene was cloned into expression vector pPIC9K, then linearized by Sac I enzyme, transformed into yeast Pichia pastoris KM17 by electroporation. The transformants with vasostatin gene integration were selected by G418+ selection, then confirmed PCR and DNA sequencing. The recombinant proteins were induced to express by methanol and purified with a yield of 12mg•L-1. CAM assay demonstrated that the yeast-expressed recombinant vasostatin obviously inhibited the angiogenesis. Production of biologically active recombinant vasostatin lays a solid foundation for further structure-function relationship study of vasostatin and its potential application.