zebularine对卵巢癌细胞的生长抑制作用-东南大学学报医学版

zebularine对卵巢癌细胞的生长抑制作用

单位：中国医科大学附属一院

分类号：

出版年·卷·期（页码）：2011·30·第四期（545-549）

摘要：

目的观察去甲基化制剂Zebularine对体外培养的卵巢癌细胞A2780的生长抑制作用，并进一步探讨其作用机制。方法应用不同浓度的Zebularine (50μmol/L, 100μmol/L,200μmol/L)处理体外培养的卵巢癌细胞A2780，应用MTT法检测细胞增殖活性；流式细胞仪检测细胞周期分布； Annexin V-FITC染色法检测细胞凋亡率。结果 100μmol/L,200μmol/L的Zebularine处理卵巢癌细胞A2780 24～72h后，细胞增殖显著受到抑制（P＜0.05）；100μmol/L,200μmol/L的Zebularine处理72h后A2780细胞G2期细胞数量显著增加（P＜0.05），S期细胞数显著降低（P＜0.05）；100μmol/L,200μmol/L浓度组细胞凋亡率均增加（P＜0.05）。结论 Zebularine对卵巢癌细胞A2780具有生长抑制作用，其机制与阻滞细胞周期及诱导凋亡有关。

Objective: To investigate the effect of Zebularine(Zeb), one of the demethylating agents, on the proliferation of human ovarian cancer cell line A2780 and its mechanism. Methods: A2780 cells cultured in RPMI1640 were treated with 50, 100, and 200μmol/L of Zeb. MTT assay, flow cytometry, and Annexin V-FITC staining were performed to analyze the cell proliferation activity, cell cycle, and apoptotic rate. Results: After A2780 cells were treated with Zeb of 100μmol/L and 200μmol/L for 24 to 72 hours, the cell proliferation activity was inhibited. The number of cells in G2 phase increased and the number of cells in S phase decreased significantly after the cells were treated with Zeb of 100μmol/L and 200μmol/L for 72 hours. The apoptotic rate increased significantly in cells treated with Zeb of 100μmol/L and 200μmol/L. Conclusion: Zeb could inhibit the growth of human ovarian cancer cell line A2780 ,which may due to block cell cycle and induce apoptosis.

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