博莱霉素致鼠肺纤维化不同时间点EGFR的表达和iressa的干预作用-东南大学学报医学版

博莱霉素致鼠肺纤维化不同时间点EGFR的表达和iressa的干预作用

作者：李龙余勤

单位：兰州大学第一医院

分类号：

出版年·卷·期（页码）：2011·30·第三期（499-502）

摘要：

目的研究肺表皮生长因子受体（EGFR）表达与肺纤维化发生的关系。方法　将54只Wistar大鼠随机分成模型组、EGFR拮抗剂Iressa组和对照组，模型组和Iressa组使用博莱霉素（5mg/kg）气管注射制造鼠肺纤维化模型, 对照组气管注入生理盐水0.2～0.3ml，造模前1h灌胃给予iressa（200mg/kg）及生理盐水10ml/kg，5次/周，分别于第1、14、28天处死大鼠。用SP免疫组化法研究原癌基因和肺EGFR在不同程度肺纤维化条件下的表达。结果　①模型组和Iressa组EGFR表达在各时间点与对照组有显著性差异；②模型组EGFR表达在第28天达到顶峰，此时间点EGFR表达与同组其他时间点均有显著性差异；③Iressa组： EGFR水平下降；同时较模型组肺纤维化程度减轻 (P<0.01)。结论　EGFR在肺纤维化不同阶段表达增强，提示EGFR可能参与肺纤维化发生过程，且可被其拮抗剂影响。

Objective　To Study Expression of epidermal growth factor receptor on pulmonary fibrosis in rats． Methods　Fifty-four Wistar rats were randomly divided into three groups, the pulmonary fibrosis group ,Iressa group and the control group, and were sacrificed after treatment on days 1 , 14 and 28 , respectively , and their lungs were harvested for histological studies. Results①The expression of EGFR in the lung significantly increased in bleomycin-treated animals(P<0.01 vs control), ②and significantly increased after 28th day in bleomycin-treated animals (P<0.01 vs other groups). ③In Iressa-treated animals， the expression of EGFR was lower than the pulmonary fibrosis group (P<0.01),④The degree of pulmonary fibrosis is alleviated in Iressa-treated animals. Conclusion　The expression of EGFR is dynamically changed in the lung with experimental pulmonary fibrosis, which may contribute to the pathogenesis of pulmonary fibrosis.

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