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奥沙利铂对子宫内膜癌裸鼠移植瘤生长及促血管生成素-2的影响
作者:孙慧婷 周怀君  
单位:东南大学医学院
关键词:奥沙利铂 子宫内膜癌 裸鼠移植瘤 促血管生成素-2 
分类号:
出版年·卷·期(页码):2011·30·第三期(464-468)
摘要:

目的:观察小剂量奥沙利铂对子宫内膜癌裸鼠移植瘤生长的抑制作用及对肿瘤组织中促血管形成素-2(Angiopoietin-2 , Ang-2)的影响。方法:体外进行Ishikawa细胞的培养扩增后种植于裸鼠皮下,建立子宫内膜癌裸鼠移植瘤模型。将20只成瘤裸鼠随机分为两组:奥沙利铂组和阴性对照组,分别应用奥沙利铂2.0mg/kg和5%葡萄糖20ml/kg进行治疗。治疗27天后测量肿瘤体积,计算抑瘤率。逆转录-聚合酶链反应(RT-PCR)和Western blot检测各组肿瘤组织中促血管生成素Ang-2的mRNA和蛋白的表达。结果:奥沙利铂组移植瘤体积458.20±77.56mm3,对照组移植瘤体积771.87±89.80mm3,二者差异有统计学意义(p<0.01)。奥沙利铂组Ang-2在mRNA及蛋白水平的表达低于对照组,二者相比差别有统计学意义(p<0.05)。结论:奥沙利铂具有抑制子宫内膜癌裸鼠移植瘤生长的作用;能抑制肿瘤组织中Ang-2的表达,可能与其抗肿瘤血管形成有关。

Objective Our observe was to determine inhibition effect of oxaliplatin on the growth of tumor transplanted from a mouse and its effect on the expression of Angiopoietin-2 of the transplanted tumor tissue.Methods After cultured and amplified in vitro, Ishikawa cells were planted into nude mice subcutaneously. When the tumor grew up to a definite size, twenty mice were randomly divided into two groups: oxaliplatin group and negative control group. The mice in each group were received corresponding treatment. After 27 days, tumor volume was assessed and tumor inhibitive rate was calculated. The expression level of Ang-2 on mRNA and protein was detected by RT-PCR and Western blotting technology respectively. Results The tumor weight in the experimental oxaliplatin group was significantly lower than that in the negative control group( P<0.01). The expression level of Ang-2 on mRNA and protein in oxaliplatin group was lower than that in the negative control group(P<0.05).Conclusion Oxaliplatin could significantly inhibit the tumor growth and the expression of Ang-2 in the mouse model of endometrial carcinoma, which may duo to the inhibitory effect of Oxaliplatin on the angiogenesis of tumor tissue.

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