AIM: To develop a pyrosequencing-based method for selection of the phage display random peptide. METHODS: After selection of Ph.D.-C7C Phage display peptide library and PCR amplification of random gene sequence of Ph.D.-C7C, the PCR products were further sequenced by pyrosequencing. RESULTS: The sequence of Ph.D.-C7C PCR products was identified by pyrosequencing ,the region are highly homologous with the motifs of GXXXHPQ , which coincides with the sequence of peptide binding to streptavidin. CONCLUSION: Pyrosequencing used in the analysis of the phage display random peptide has the advantage of high resolution and can be widely employed in sequencing of the phage display random peptide library. |