铜绿假单胞菌注射液与5-Fu腹腔化疗对大鼠结肠吻合口愈合影响的对比研究-东南大学学报医学版

铜绿假单胞菌注射液与5-Fu腹腔化疗对大鼠结肠吻合口愈合影响的对比研究

单位：辽宁医学院第一附属医院

分类号：

出版年·卷·期（页码）：2011·30·第二期（355-359）

摘要：

【摘要】　目的：比较铜绿假单胞菌注射液与5-Fu腹腔化疗对大鼠结肠吻合口愈合的影响并研究其机制。方法：40只SD大鼠，随机均分为4组，每组10只，均行结肠部分切除再吻合术。A：模型对照组；B：铜绿假单胞菌注射液（皮下注射）组；C：铜绿假单胞菌注射液（皮下注射+术中腹腔喷洒）组；D：5-Fu（腹腔化疗）组。术后7天检测吻合口爆破压、病理学形态、胶原纤维含量、转化生长因子（TGF-β）及纤维连接蛋白（FN）表达。结果：B组和C组脏器粘连程度最低，吻合口破裂压、炎性细胞数量、胶原纤维沉积、及TGF-β和FN表达显著高于A组和D组。结论：铜绿假单胞菌注射液可提高机体免疫状态，增加吻合口炎性细胞数量，促进结肠吻合口早期愈合，降低吻合口漏的发生，还可减轻瘢痕生成，降低粘连程度。

【Abstract】 Objective: To compare the effection of Pseudomonas aeruginosa injection on rat colonic anastomotic healing with 5-Fu intraperitoneal chemotherapy, study its mechanism. Methods: 40 SD rats were randomly divided into 4 groups with 16 rats for each, underwent colon resection and anastomosis. A: control group; B: Pseudomonas aeruginosa injection (subcutaneous injection) group; C: Pseudomonas aeruginosa injection (subcutaneous + peritoneal spraying) group; D: 5-Fu (intraperitoneal chemotherapy) group. 7 days after detection of anastomotic bursting pressure, pathology, collagen fiber content, transforming growth factor (TGF-β) and fibronectin protein。Results: B group and C group the lowest level of visceral adhesions, anastomotic bursting pressure, inflammatory cells, collagen deposition, and TGF-β and FN was significantly higher than the A group and D group. Conclusion: Pseudomonas aeruginosa injection can improve the immune status, increased number of anastomotic inflammatory cells and promote early healing of colonic anastomosis, reducing the incidence of anastomotic leakage, can reduce scar formation and reduce the extent of adhesions.

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