Abstract: Confirm the MHC class I molecules (H2-K and H2-Qa1) expression in the primary cultured mouse cortical neurons. In order to make double transgenic mouse over-expression functional MHC class I complexes specifically in the hippocampus, H2-K(flag) and β2-m were subcloned into plasmid pBlueScript-Frizzled9-promoter at the downstream of this hippocampal-specific promoter.. Three strains of H2-K/β2-m double transgenic mouses and two strains of β2-m transgenic mouses were generated now and ready for the further studying of MHC’s role in nerve system.