壳聚糖修饰单壁碳纳米管的制备与细胞毒性研究-东南大学学报医学版

壳聚糖修饰单壁碳纳米管的制备与细胞毒性研究

单位：南京师范大学

分类号：

出版年·卷·期（页码）：2011·30·第一期（52-56）

摘要：

目的：制备稳定的水溶性的壳聚糖/碳纳米管（CHI/SWCNT-COOHs）纳米材料以及对其表征，并检测纳米材料的细胞毒性。方法：市售单壁碳纳米管(Raw SWCNTs)以强酸处理, 形成残缺的羧基化单壁碳纳米管(SWCNT-COOHs)，然后，低分子壳聚糖(CHI)与SWCNT-COOHs反应制备壳聚糖/碳纳米管（CHI/SWCNT-COOHs）纳米材料；运用透射电镜技术、拉曼光谱技术检测纳米材料的性状；通过台盼蓝染色计算纳米材料对A549细胞存活率的影响，以及计算纳米材料营养耗竭后对单细胞克隆的影响，运用扫描电镜检测对红细胞形态的影响。结果：CHI/SWCNT-COOHs在水系中很好的稳定性，对细胞无明显毒性，营养耗竭量减少，对红细胞形态没有显著影响。结论： CHI/SWCNT-COOHs性状稳定，具有纳米特性，细胞毒性低。

Objective: Used SWCNTs and CHI to synthesis CHI/SWCNT-COOHs as nano-materials, and tested it’s cells toxicity.Methods: Raw single walled carbon nanotubes (SWCNTs) were treated by acid oxidation to form Carboxylic SWCNT-COOHs. The amino of low molecular weight chitosan (CHI) and the carboxyl of SWCNT-COOHs formed nano-materials CHI / SWCNT-COOHs. The characters CHI/SWCNT-COOHs were determinated by transmission electron microscopy (TEM), Raman spectroscopy.A549 cells were treated with carbon nanotubes, and used Trypan blue cell viability assay test; Single-cell colonies statistics after CNTs nutrient depletion one week; Morphology Structural of the red blood cell by TEM with CNTs treated. Results: CHI/ SWCNT-COOHs with good stability in water systems improved the survival rate of cells, reduced nutrient depletion, and no significant effect on red blood cell morphology. Conclusion: CHI/SWCNT-COOHs had a stability in water, a nano-features, and low cytotoxicity.

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