Abstract: Objective: to investigate the effect of spironlactone on the expression of endothelial nitric oxide synthase(e-NOS) of rabbit coronary artery in the area of noninfarcted and hemodynamics of cardiovascular in early period of acute myocardial infraction(AMI). Methods:24 male rabbits were divided into three groups(A,B,C)randomly. Rabbits in group A(n=8) and B(n=8) were both feeded with high fat diet for four weeks, and those in group A were feeded with spironolactone（20mg/kg/d）at the same time. The middle and distal segment of anterior descending branch of all rabitts in group A and B were ligated to make AMI model,and animals in group C weren’t deligated. The level of aldosterone (Aldo) and expression of endothelial nitric oxide synthase (e-NOS) in coronary endotheliocyte which located near the spot of deligation were determinated fifteen minutes postoperation. Mean systolic pressure(MSP) ,mean diastolic pressure(MDP), mean aterial blood pressure(MAP) and isovolumic contraction / relaxation function（+dp/dt max、-dp/dt max）of left ventricular were measured and compared, by the way of making carotid arterial cannulation. Results: the expression of e-NOS in coronary endotheliocyte that hadn’t been ligated in group A and B were both more weaker than that in group C, and that in group A were better than that in group B(P<0.05). The level of Aldo in group A and B was much higher than that in group C(P<0.05),and there were no significant difference between than that in group A and group B. The change of MSP、MDP、MAP and +dp/dt max of left ventricular in group A and B were much higher than that in group C, and the changes of MSP and +dp/dt max of left ventricular in group A were higher than that in group C. Conclusion: Spironolactone could reinforce the expression of e-NOS in coronary endotheliocyte that hadn’t been deligated, and high fat feeding could decrease it. Spironolactone could improve the hemodynamics state of heart in pristine period of AM