Objective To Investigate the Inhibition affects and the mechanism of tetramethylpyrazine on angiogenesis of solid tumor in mice. Methods Established the model of colorectal cancer sw620 xenograft in nude mice,they were randomly divided into five groups: NS group,the low, middle and high dose of tetramethylpyrazine group,Endostar group. Measuring tumor volume and weight after administration.Observed pathological changes in tumors .Immunohistochemistry and Western blot was used to detect tumor tissue expression of CD34, VEGF, HIF-1α. Results Compared with the NS group,colorectal cancer sw620 xenografts in the middle and high dose of tetramethylpyrazine grope significantly reduced the size and weight. CD34, VEGF, HIF-1α expression was significantly lower in the tumor.Conclusion Tetramethylpyrazine can inhibit colorectal cancer sw620 xenografts growth in nude mice,the mechanism of affect may be related to to improve the status of tumor tissue hypoxia and Inhibit tumor angiogenesis.