组蛋白HIST1H2AC 和HIST1H2BC基因在胰腺癌耐药细胞株中的表达-东南大学学报医学版

组蛋白HIST1H2AC 和HIST1H2BC基因在胰腺癌耐药细胞株中的表达

单位：东南大学

关键词：组蛋白 HIST1H2AC HIST1H2BC 胰腺癌细胞株Puta8988 培美曲塞

分类号：

出版年·卷·期（页码）：2010·29·第五期（490-493）

摘要：

[目的] 研究组蛋白HIST1H2AC和HIST1H2BC在培美曲塞(pemetrexed)诱导的胰腺癌耐药细胞株Puta8988中的基因和蛋白表达水平，以明确HIST1H2AC和HIST1H2BC基因与胰腺癌耐药的关系。 [方法] 以两种剂量的培美曲塞诱导胰腺癌细胞株Puta8988，使其形成两个等级的获得性耐药细胞株(Puta8988+、Puta8988++)。采用qRT-PCR和Western blot方法分别检测HIST1H2AC和HIST1H2BC在耐药细胞株及其亲本（对照）中的基因和蛋白的表达水平。 [结果] qRT-PCR检测结果显示，组蛋白HIST1H2AC和HIST1H2BC基因在胰腺癌耐药株Puta8988+和Puta8988++中的表达水平显著上调（P<0.05），其中HIST1H2AC基因比对照分别上调4.64倍和5.88倍，HIST1H2BC基因比对照分别上调5.67倍和23.07倍。Western blot检测结果显示，组蛋白HIST1H2AC和HIST1H2BC在胰腺癌耐药株Puta8988+和Puta8988++中的蛋白表达水平也显著上调（P<0.05），其中HIST1H2AC蛋白比对照分别上调54.35％和87.43％，HIST1H2BC基因比对照分别上调189.26％和774.85％。 [结论] 胰腺癌细胞株经培美曲塞诱导后，其组蛋白HIST1H2AC和HIST1H2BC在基因和蛋白表达水平上均出现上调，提示HIST1H2AC和HIST1H2BC基因可能与耐药有关。 [关键词] 组蛋白；HIST1H2AC；HIST1H2BC；胰腺癌细胞株Puta8988；培美曲塞

[Objective] To investigate the expression of HIST1H2AC and HIST1H2BC genes in pancreatic cancer cell lines Puta8988 treated with Pemetrexed, and verify the relationship between the genes and Pemetrexed-resistance. [Method] Pancreatic cancer cell lines Puta8988 were treated with two doses of Pemetrexed to get Puta8988+ and Puta8988++ with Pemetrexed-resistance, the expression levels of HIST1H2AC and HIST1H2BC and their genes in in Puta8988, Puta8988+ and Puta8988++ were detected by qRT-PCR and Western blot respectively. [Result] Results detected by qRT-PCR showed that expression levels of HIST1H2AC and HIST1H2BC genes were significantly up-regulated in Puta8988+ and Puta8988++ (P<0.05), for HIST1H2AC, up-regulated range was 4.64 times and 5.88 times of Puta8988 (control) respectively, for HIST1H2BC, 5.67 times and 23.07 times, respectively. Results detected by Western blot showed that expression levels of HIST1H2AC and HIST1H2BC were significantly up-regulated too in those cell lines with Pemetrexed-resistance (P<0.05), compared to control, the HIST1H2AC increased 54.35％ and 87.43％ in in Puta8988+ and Puta8988++, respectively, the HIST1H2BC did 189.26％ and 774.85％, respectively. [Conclusion] That expressions of HIST1H2AC and HIST1H2BC and their genes in pancreatic cancer cell lines Puta8988 treated with Pemetrexed were significantly up-regulated, which implies that these genes possibly plays a role in Pemetrexed-resistance of pancreatic cancer cell lines. [Key words] HIST1H2AC HIST1H2BC; pancreatic cancer; Puta8988; Pemetrexed

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