Objective To discuss the method of the primary culture and identification of mouse renal tubular epithelial cells,and supply a experimental platform for the rearch of renal stones.Methods Mechanical grinding and Collagenase Ιdigestion were used to isolate renal tubular segments,the collected cells were cultured in incubation with 5%CO2 at 37℃,using the EpiCM with 10%FBS and 1% EpicGs.The cells were subcultured after digestion with 0.25% trypsin.The cells were identified by Immunocytochemistry.Results After 3 days,the cultured cells were completely adhered,the logarithmic growth phase was beween 4 and 7 days. The cells were large,displaying the typical cobblestone appearence,and successfully identified by Immunocytochemistry using cytokeratin 18. Conclusions Mechanical grinding and Collagenase Ιdigestion combined with EpiCM is an ideal method to collect and culture the renal tubular epithelial cells,and it provides the experimental basis for further studing the etiology and mechanism of urinary tract stones.