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光子晶体微载体三维培养平台用于HMSN复合载药系统抗肿瘤效果的初步鉴定
作者:郭欣1  郭楠2  蔡云朗3  徐延华1 
单位:1. 济南市妇幼保健院 妇科, 山东 济南 250000;
2. 山东省第一医科大学附属省立医院 药学部, 山东 济南 250000;
3. 东南大学附属中大医院 妇产科, 江苏 南京 210009
关键词:光子晶体微载体 复合载药系统 三维培养 卵巢癌 
分类号:R737.31;R453
出版年·卷·期(页码):2022·41·第五期(644-651)
摘要:

目的:通过光子晶体微载体构建三维仿生肿瘤细胞培养平台,初步检测此平台的药物筛选作用及HMSN-COOH@DOXfluorescence NVP复合载药系统在三维培养环境中对CD117+CD44+A2780和A2780细胞的化疗效果。方法:于常规二维培养及三维仿生平台分别接种CD117+CD44+A2780和A2780细胞,加入DOX、PTX、CDDP和5-FU,分别计算各组的抑制率及凋亡率,验证三维仿生平台的可行性。将HMSN复合载药系统(包载DOX)加入各组,对照组加入相同剂量的游离药物,共孵育12 h后检测细胞凋亡率。结果:光子晶体微载体为细胞提供了仿生的三维生长环境。三维培养平台中DOX、PTX、CDDP及5-FU对CD117+CD44+A2780和A2780细胞的化疗效果显著降低(P<0.05)。HMSN复合载药系统对在三维和二维培养环境中的细胞均有较好的促凋亡作用,等量游离药物对三维和二维环境中培养细胞的促凋亡作用均小于HMSN复合载药系统(P<0.05)。结论:本实验构建的光子晶体微载体可为肿瘤细胞的生长提供良好的仿生三维环境,并可用于化疗药物的初步筛选,HMSN复合载药系统对三维培养环境中的卵巢癌细胞仍可发挥良好的抗肿瘤效果,为动物实验结果的预期和在体实验的进行提供了有力的依据。

Objective: To construct a three-dimensional biomimetic platform by using photonic crystal microcarriers for tumor cell culture, and the drug screening effect of this platform was preliminarily tested. And also the chemotherapeutic effect of the HMSN compound drug delivery system,which established by our laboratory, on CD117+ CD44+ A2780 and A2780 cells in this three-dimensional culture environment was preliminarily studied. Methods: CD117+ CD44+ A2780 and A2780 cells were respectively inoculated on conventional and three-dimensional bionic platforms, then DOX, PTX, CDDP and 5-FU were added,the inhibition rate and apoptosis rate of each group were calculated respectively to verify the three-dimensional applicability of bionic platforms. HMSN compound drug delivery system (loaded with DOX) were added to each group, the same dose of free drug was added to the control group, the apoptotic rate of each group was detected after co-incubating for 12 hours. Results: The chemotherapeutic effects of DOX, PTX, CDDP and 5-FU on CD117+ CD44+ A2780 and A2780 cells in the three-dimensional culture platform were significantly decreased (P<0.05), which indicated that the photonic crystal microcarriers could provide a biomimetic three-dimensional growth environment for the tumor cells. The pro-apoptotic effects of the same amount of free drugs on cultured cells in three-dimensional and conventional environments were significantly lower than those in the HMSN compound drug delivery system (P<0.05), prove that the HMSN compound drug delivery system may have better in vivo effects. Conclusion: The photonic crystal microcarriers constructed in this study can provide a biomimetic three-dimensional environment for the growth of tumor cells, which can be used for the preliminary screening of chemotherapeutic drugs, the HMSN compound drug delivery system exhibits a good antitumor effect on ovarian cancer cells in the three-dimensional culture environment, which provides strong basis for future animal and in vivo experiments.

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