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免疫组化染色法检测结直肠癌MMR蛋白表达的时效性分析
作者:曹晓卉  杨志慧  梅佳  殷正进  张缨 
单位:东部战区总医院(原八一医院)病理科, 江苏 南京 210002
关键词:免疫组化染色 结直肠癌 错配修复蛋白 检测时效 
分类号:R446.8
出版年·卷·期(页码):2019·38·第六期(1063-1065)
摘要:

目的:通过免疫组化法检测分析DNA错配修复(mismatch repair,MMR)蛋白在结直肠癌标本中表达的时效性,旨在探讨结直肠癌术后标本行MMR检测的有效时间。方法:选取东部战区总医院(原八一医院)2010年1月至2018年12月结直肠癌手术标本45例,每例均设置阳性对照后进行MMR蛋白(MLH1、MSH2、MSH6和PMS2)的免疫组化检测。结果:在阳性对照均正常表达的情况下,MMR蛋白免疫组化检测结果显示,2010、2011年的标本着色较弱,2012年标本着色有所减弱,2013年至2018年标本的着色程度基本相同。结论:MMR蛋白缺失与结直肠癌的发生、发展密切相关,选取8年内的病理组织学蜡块切片进行免疫组化检测更具有效性。

Objective: To detect and analyze the time-effect of expression of DNA mismatch repair (MMR) protein in colorectal cancer through immunohistochemical method and discuss the effective time of MMR detection in colorectal cancer specimens after operation.Methods: A total of 45 cases of colorectal cancer surgical specimens from January 2010 to December 2018 from the General Hospital of the Eastern Theater Command (formerly Bayi Hospital) were selected.Each case was set up with a positive control for MMR protein (MLH1, MSH2, MSH6 and PMS2), then immunohistochemical detection was perfomed. Results: In the case of normal expression of the positive control, the MMR protein immunohistochemical test showed that the specimens were weakly stained in 2010 and 2011, the specimens were weakened in 2012, and that the staining degree was basically the same from 2013 to 2018. Conclusion: The absence of MMR protein is closely related to the occurrence and development of colorectal cancer, and it is more effective to select pathologic wax slices within 8 years for immunohistochemical detection.

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