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骨肉瘤中eIF2α和VEGF的表达及其意义
作者:侯威  张卫  贺洪辉  向亮 
单位:南华大学附属南华医院 关节运动科, 湖南 衡阳 421000
关键词:骨肉瘤 真核翻译起始因子2α 血管内皮生长因子 预后 
分类号:R733.3
出版年·卷·期(页码):2019·38·第五期(774-780)
摘要:

目的:观察骨肉瘤组织中真核翻译起始因子2α(eIF2α)和血管内皮生长因子(VEGF)蛋白及mRNA的表达,探讨其临床意义。方法:收集61例骨肉瘤患者作为研究组1,骨巨细胞瘤患者41例作为对照组1,因外伤切除正常骨组织的患者41例作为正常对照组,均留取术后石蜡包埋组织;收集21例骨肉瘤新鲜组织作为研究组2,21例骨巨细胞瘤新鲜组织作为对照组2。应用免疫组化方法检测研究组1、对照组1和正常对照组中eIF2α和VEGF蛋白的表达,应用蛋白质印迹法检测研究组2和对照组2中eIF2α和VEGF的半定量表达,应用荧光PCR技术检测研究组2和对照组2中eIF2α和VEGF mRNA的表达。结果:免疫组化结果显示,研究组1、对照组1和正常对照组中eIF2α和VEGF表达的阳性率差异有统计学意义(P<0.05)。研究组1中eIF2α和VEGF蛋白的表达均与病变级别、周围组织累犯和肿瘤最大径有关(P<0.05),eIF2α蛋白的表达还与增殖指数有关(P<0.05),VEGF蛋白的表达还与转移有关(P<0.05)。蛋白质印迹法检测结果显示,研究组2和对照组2中eIF2α和VEGF的半定量表达差异有统计学意义(P<0.05)。荧光PCR结果显示,研究组2和对照组2中eIF2α和VEGF mRNA的表达差异有统计学意义(P<0.05)。研究组1中eIF2α蛋白与VEGF蛋白表达呈正相关(r=0.56,P=0.017 0),生存分析显示研究组1中eIF2α和VEGF蛋白的表达与生存时间相关(P<0.05)。结论:骨肉瘤组织中eIF2α和VEGF的蛋白及mRNA表达明显升高,二者呈正相关,共同促进病变的形成和进展。eIF2α和VEGF的表达与预后有关。

Objective: To observe the expressions of eukaryotic translation initiation factor 2α(eIF2α) and vascular endothelial growth factor(VEGF) protein and mRNA in osteosarcoma, explore their correlation and clinical significance. Methods: 61 cases of osteosarcoma were collected as study group1, 41 cases of giant cell tumor of bone were collected as control group1, 41 cases of normal bone tissue(trauma) were collected as normal control group. Paraffin-embedded tissues were retained after operation. 21 cases of fresh osteosarcoma were collected as study group2, 21 cases of giant cell tumor fresh tissue of bone were collected as control group2. Expressions of eIF2α and VEGF protein were detected in study group1, control group1 and normal control group by immunohistochemistry. Semi-quantitative expression of eIF2α and VEGF protein were detected in study group2 and control group2 by Western blotting method. Expression of eIF2α and VEGF mRNA were detected in study group 2 and control group 2 by fluorescent PCR. Results: Immunohistochemical results showed that positive rates of eIF2α and VEGF protein were significantly different in study group1, control group1 and normal control group(P<0.05). In study group1, Expression of eIF2α and VEGF protein were all correlated with pathological grade, surrounding tissue recidivism and tumor maximum diameter, expression of eIF2α protein was also correlated with proliferation index, expression of VEGF protein was also correlated with metastasis. Western blotting results showed that semi-quantitative expression of eIF2α and VEGF were significantly different between study group2 and control group2(P<0.05). Fluorescence PCR results showed that expression of eIF2α and VEGF mRNA were significantly different between study group2 and control group2(P<0.05). There was a positive correlation between eIF2α protein and VEGF protein in study group1. Survival analysis showed that eIF2α and VEGF protein were correlated with survival time in study group1. Conclusion: Expression of eIF2α and VEGF protein and mRNA is significantly higher in osteosarcoma tissues. eIF2α and VEGF are positively correlated. The expression of eIF2α and VEGF is associated with prognosis of osteosarcoma.

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