Objective:To study the effect of thymopentin on the immunologic function and Toll like recptor-4(TLR-4) signal pathway in the spleen in mouse model of lung cancer. Methods:Forty mice of C57BL/6 were randomly divided into normal control(NC) group, model control(MC) group, cyclophosphamide(CPM) group and thymopentin(THY) group. Except NC group, mice in other three groups were given subcutaneously Lewis lung cancer cells under right armpit to make mouse model of lung cancer. After two-week treatment, regulatory T cell(Treg cell) and T help cell 17(Th17 cells) were determined, and mRNA and protein of TLR4, tumor necrosis factor receptor-associated factor-6(TRAF-6), nuclear factor-κB (NF-κB) and activating protein-1(AP-1) were determined by transcription-polymerase chain reaction and Western-blotting. Results:(1)Compared with CPM group, the level of Treg and Th17 cells in THY group were increased significantly(P<0.01), and the ratio of Treg/Th17 was decreased significantly(P<0.01). (2)Compared with CPM group, the mRNA and protein level of TLR4, TRAF-6, NF-κB and AP-1 in the spleen of THY group were decreased significantly(P<0.01). Conclusion:Adjuvant therapy of thymopentin in the chemotherapy of cyclophosphamide for the mice with lung cancer can regulate the immunologic function and down-regulate the level of TLR4, TRAF-6, NF-κB and AP-1 in the TLR4 signal pathway in the spleen.
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