Objective:To study the mechanism of levotinib regulating VEGFR/NF-κB/R1P2 signaling in primary hepatocellular carcinoma. Methods:Eighty patients with primary liver cancer admitted to our hospital from March 2016 to October 2017 were enrolled in the study. The patients were treated with oral levoftenib 24 mg daily until the disease progressed and intolerance was unacceptable. Drug efficacy and adverse reactions were evaluated in patients; immunohistochemistry was used to detect the expression of VEGFR2 protein in cancer tissues; Real-Time PCR and Western Blot were used to detect NF-κB, R1P2, BCL-2, BCL-xl and Caspase3 mRNA expression and phosphorylated protein levels. Results:The effective rate of Lefatinib in the treatment of primary hepatocellular carcinoma was 57.50%. Compared with paracancerous tissues, the expression of NF-κB, R1P, BCL-xl, Caspase3 mRNA and protein in primary hepatocellular carcinoma was significantly increased, and the contents of VEGFR2, BCL-2 mRNA and protein were significantly decreased after lovastinib was treated with Lefatinib. The difference was statistically significant (P<0.05). Conclusion:Levitinib regulates the expression of VEGFR/NF-κB/R1P2 in hepatocellular carcinoma and promotes the apoptosis of hepatoma cells. The incidence of adverse reactions of levabinib in the treatment of primary liver cancer is low, and the clinical application is safe. |
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