目的：探讨枸杞多糖（LBP）对H₂O₂诱导人视网膜色素上皮细胞（ARPE-19细胞）自噬及对Beclin-1、LC3B表达的影响。方法：将ARPE-19细胞用不同浓度H₂O₂处理后应用透射电镜观察H₂O₂对ARPE-19细胞自噬的影响，蛋白质印迹法检测不同浓度的LBP对H₂O₂诱导ARPE-19细胞Beclin-1、LC3BⅠ和LC3BⅡ表达的影响。结果：H₂O₂诱导ARPE-19细胞12 h后，与对照组比较，H₂O₂ 600 μmol·L^-1组自噬表达显著增加。H₂O₂诱导后，ARPE-19细胞Beclin-1和LC3BⅠ、LC3BⅡ表达显著升高，LC3BⅡ/LC3BⅠ值升高（P<0.01）。1 000 μg·mL^-1LBP+400 μmol·L^-1H₂O₂组Beclin-1、LC3BⅠ和LC3BⅡ表达显著降低（P<0.01）。结论：枸杞多糖能够降低Beclin-1、LC3BⅠ和LC3BⅡ表达，干预H₂O₂诱导所致ARPE-19细胞的自噬水平升高，干预效应在浓度为1 000 μg·mL^-1时最为显著。

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