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慢性疼痛对孕鼠及其子代认知功能的影响
作者:周芳1  李清1  闫文靖1  刘玉平2  涂淑敏3 
单位:1. 徐州医科大学 护理学院, 江苏 徐州 221004;
2. 徐州医科大学附属医院 护理部, 江苏 徐州 221002;
3. 北京清华长庚医院 麻醉科, 北京 102218
关键词:慢性疼痛 孕鼠 子鼠 认知功能 
分类号:R-332
出版年·卷·期(页码):2018·37·第六期(985-989)
摘要:

目的:通过构建孕期小鼠慢性疼痛模型,观察慢性疼痛对孕鼠及子鼠认知功能的影响。方法:将交配成功的母鼠随机分为正常组、假手术组和慢性疼痛组,每组10只。慢性疼痛组母鼠足跖关节皮内注射无菌完全弗氏佐剂100 μl(即500 μg),诱导产生关节炎性痛。用水迷宫实验检测动物认知能力。结果:定位航行实验中慢性疼痛组孕鼠平均逃避潜伏期比假手术组长(P<0.001)。空间搜索实验中慢性疼痛组孕鼠穿越平台次数、平均游速、在原平台象限停留的时间比率及游程比率均低于假手术组(P<0.05)。子鼠出生后第3周,慢性疼痛组子鼠平均逃避潜伏期比假手术组长(P<0.01),穿越平台次数少于假手术组子鼠(P<0.05),平均游速、在原平台象限停留的时间比率和游程比率与假手术组子鼠比较,差异无统计学意义(P>0.05)。结论:孕期慢性疼痛会导致母体认知能力的下降,也会影响其子代幼年期的认知功能。

Objective:To observe the effects of chronic pain on the cognitive function of pregnant mice and their offspring by constructing the animal model of chronic pain in pregnant mice. Methods:The pregnant mice were divided into normal group, sham-operation group and chronic pain group (10 in each group). Aseptic complete Freund's adjuvant 100 μl (500 μg) was injected intracutaneously in the paw joint of pregnant mice to induce arthritis pain. Water labyrinth experiment was performed to evaluate the cognitive ability of the animals. Results:In the place navigation test, the average escape latency of the mice in chronic pain group were longer than that in sham operation group (P<0.001).In the spatial search experiment, the number of times of crossing the platform, the average swimming speed, the swimming time ratio and the swimming distance ratio staying in the original platform quadrant of the mice in the chronic pain group were less than those in the sham operation group (all P<0.05).At the third week after their offspring birth, the average escape latency of offspring mice in the chronic pain group was longer than that in the sham operation group(P<0.01), and the number of times of crossing the platform was less than that in the sham operation group (P<0.05).There was no difference in the average swimming speed, the swimming time ratio and the swimming distance ratio staying in the original platform quadrant between the offspring mice of the two groups (P>0.05). Conclusions:Chronic pain during pregnancy can lead to a decline in maternal cognitive ability and also affect the cognitive function of the offspring in infancy.

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