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改良镇静技术促进鸡胚荷瘤模型的磁共振成像研究
作者:左智1  左鹏飞1  王鑫1  史秋寅1  丁建东1  左扬松2  马根山1 
单位:1. 东南大学附属中大医院 心血管内科, 江苏 南京 210009;
2. 涟水县人民医院 呼吸内科, 江苏 涟水 223400
关键词:鸡胚荷瘤模型 磁共振 乳腺癌细胞异体移植模型 肿瘤成像 冷却镇静 
分类号:R73-354;R445.2
出版年·卷·期(页码):2018·37·第五期(835-840)
摘要:

目的:改良冷却镇静技术,提高鸡胚荷瘤模型的磁共振成像(MRI)质量,拓宽其影像学分析的可能性。方法:使用44只鸡胚,其中29只用于改良冷却镇静技术、优化MRI序列。15只构建鸡胚荷乳腺癌模型,MDA-MB-231细胞移植后4~9 d使用MRI分析肿瘤生长。扫描完采集实体肿瘤组织,与MRI所得数据比对,评价该模型MRI成像的精度与可靠度。结果:成功总结出改良的镇静技术,使孵育7~18 d的鸡胚维持镇静达90 min,这样的时间长度可满足多数MRI扫描所需。获得的图像像素最高可达77 μm×91 μm,对于肿瘤的MRI评估无论是大小还是内部结构都非常可靠。结论:本研究证实了改良后的冷却镇静技术可以提高鸡胚荷瘤模型的MRI成像效果,进一步拓宽了该模型的应用范围,带来了更多的可能性。

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